ArticleInfection and immunity2000
Adherence of Staphylococcus aureus to endothelial cells: influence of capsular polysaccharide, global regulator agr, and bacterial growth phase.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed.
- Key role for clumping factor B in Staphylococcus aureus nasal colonization of humans.PLoS medicine · 2008Trial
- Potential Compounds as Inhibitors of Staphylococcal Virulence Factors Involved in the Development of Thrombosis.Toxins · 2025Review
- The endothelium at the interface between tissues andClinical microbiology reviews · 2025Review
- Bacterial capsules: Occurrence, mechanism, and function.NPJ biofilms and microbiomes · 2024Review
- Limited Adaptation ofMicrobiology spectrum · 2023Article
- On-person adaptive evolution of Staphylococcus aureus during treatment for atopic dermatitis.Cell host & microbe · 2023Article
- A genetic regulatory see-saw of biofilm and virulence in MRSA pathogenesis.Frontiers in microbiology · 2023Review
- Regulation of Staphylococcal Capsule by SarZ is SigA-Dependent.Journal of bacteriology · 2022Article
- Antibiotic Resistance and Pathogenomics ofMicroorganisms · 2021Article
- Staphylococcus aureus in Agriculture: Lessons in Evolution from a Multispecies Pathogen.Clinical microbiology reviews · 2021Review
- MgrA Activates Staphylococcal Capsule via SigA-Dependent Promoter.Journal of bacteriology · 2020Article
- Revealing 29 sets of independently modulated genes inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors.Molecular microbiology · 2020Article
- MgrA Negatively Impacts Staphylococcus aureus Invasion by Regulating Capsule and FnbA.Infection and immunity · 2019Article
- Staphylococcus aureus adhesion in endovascular infections is controlled by the ArlRS-MgrA signaling cascade.PLoS pathogens · 2019Article
- Coordination of capsule assembly and cell wall biosynthesis in Staphylococcus aureus.Nature communications · 2019Article
- Distinct phenotypic traits of Staphylococcus aureus are associated with persistent, contagious bovine intramammary infections.Scientific reports · 2018Article
- Pathogen interactions with endothelial cells and the induction of innate and adaptive immunity.European journal of immunology · 2018Review
- Survey of potential factors involved in the low frequency of CP5 and CP8 expression in Staphylococcus aureus isolates from mastitis of dairy cattle from Argentina, Chile, and Uruguay.Journal of applied genetics · 2018Article
- Article
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Authors and funding
8 authors.
Funding
Abstract
The adherence of Staphylococcus aureus to human endothelial cells (EC) is probably an important step in the pathogenesis of systemic staphylococcal infections. We examined the influence of type 5 capsular polysaccharide (CP5) production, the global regulator agr, and the bacterial growth phase on S. aureus adherence to EC. Whereas S. aureus Newman showed maximal adherence to EC in the logarithmic phase of growth, an isogenic agr mutant showed maximal adherence in the stationary growth phase. S. aureus adherence to EC and CP5 expression were negatively correlated: a mutation in the agr locus diminished CP5 production and led to increased adherence. Likewise, induction of CP5 expression by addition of NaCl to the growth medium resulted in reduced staphylococcal adherence to EC. S. aureus Newman cells that adhered to EC did not express CP5. A Newman cap5O mutant was acapsular and showed significantly greater adherence to EC than the parental strain did (P<0.005). Complementation of the cap5O mutation in trans restored CP5 expression and reduced EC adherence to a level similar to that of the parental strain. The enhanced adherence shown by the cap5O mutant was similar in magnitude to that of the agr mutant or the cap5O agr double mutant. Cells of the cap5O mutant and cap5O agr double mutant harvested from stationary-phase cultures adhered significantly better than did cells harvested in the exponential growth phase. These data are consistent with the postexponential and agr-independent expression by S. aureus of at least one putative EC adhesin, whose binding domain may be masked by CP5.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.