ArticleThe Biochemical journal2000
A role for serine-175 in modulating the molecular conformation of calponin.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Mechanoregulation and function of calponin and transgelin.Biophysics reviews · 2024Review
- Evolution and function of calponin and transgelin.Frontiers in cell and developmental biology · 2023Review
- A rapid degradation of calponin 2 is required for cytokinesis.American journal of physiology. Cell physiology · 2021Article
- Label-free quantitative phosphorylation analysis of human transgelin2 in Jurkat T cells reveals distinct phosphorylation patterns under PKA and PKC activation conditions.Proteome science · 2015Article
- Distinct conformational and functional effects of two adjacent pathogenic mutations in cardiac troponin I at the interface with troponin T.FEBS open bio · 2015Article
- Cytoskeletal reorganization evoked by Rho-associated kinase- and protein kinase C-catalyzed phosphorylation of cofilin and heat shock protein 27, respectively, contributes to myogenic constriction of rat cerebral arteries.The Journal of biological chemistry · 2014Article
- New insights into the functional significance of the acidic region of the unique N-terminal extension of cardiac troponin I.Biochimica et biophysica acta · 2013Article
- Alanine or aspartic acid substitutions at serine23/24 of cardiac troponin I decrease thin filament activation, with no effect on crossbridge detachment kinetics.Archives of biochemistry and biophysics · 2012Article
- The heart-specific NH2-terminal extension regulates the molecular conformation and function of cardiac troponin I.American journal of physiology. Heart and circulatory physiology · 2012Article
- Transgenic analysis of the role of FKBP12.6 in cardiac function and intracellular calcium release.Assay and drug development technologies · 2011Article
- A high-throughput solid-phase microplate protein-binding assay to investigate interactions between myofilament proteins.Journal of biomedicine & biotechnology · 2011Article
- Pressure-dependent contribution of Rho kinase-mediated calcium sensitization in serotonin-evoked vasoconstriction of rat cerebral arteries.The Journal of physiology · 2010Article
- h3/Acidic calponin: an actin-binding protein that controls extracellular signal-regulated kinase 1/2 activity in nonmuscle cells.Molecular biology of the cell · 2010Article
- Ca2+ sensitization via phosphorylation of myosin phosphatase targeting subunit at threonine-855 by Rho kinase contributes to the arterial myogenic response.The Journal of physiology · 2009Article
- To investigate protein evolution by detecting suppressed epitope structures.Journal of molecular evolution · 2009Article
- Direct association of calponin with specific domains of PKC-alpha.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Microtiter plate monoclonal antibody epitope analysis of Ca2+- and Mg2+-induced conformational changes in troponin C.Archives of biochemistry and biophysics · 2007Article
- Cytoskeletal tension regulates both expression and degradation of h2-calponin in lung alveolar cells.Biochemistry · 2006Article
- Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension.Gut · 2002Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calponin is an actin filament-associated protein found in smooth muscle and non-muscle cells. Calponin inhibits actin-myosin interaction in a manner that is prevented by protein kinase C (PKC)-catalysed phosphorylation of serine-175. To investigate the molecular basis of serine-175-mediated regulation, we examined the effect of phosphorylation on the conformation of calponin using monoclonal antibody (mAb) epitope analysis. Eight mAbs against different epitopes on chicken gizzard calponin were developed to monitor the conformational changes in calponin induced by PKC-mediated phosphorylation or serine-175-->alanine (S175A) substitution. The relative affinities of the mAbs for calponins immobilized on microtitre plates or bound to actin-tropomyosin thin filaments were determined, and epitope competitions between free and immobilized calponins were carried out. The changes in binding affinity between mAb paratopes and calponin epitopes demonstrate several serine-175 modification-induced conformational effects: (a) structures of calponin are reconfigured by serine-175 modification, supporting the regulatory function of serine-175; (b) there are submolecular structures unaffected by modification of serine-175 in both free and thin filament-associated calponins, suggesting that the serine-175-based conformational modulation is a targeted allosteric effect; (c) significant conformational changes are detected between free and thin filament-associated calponins, indicating two functional states of the molecular conformation; and (d) the different epitope characteristics between thin filament-bound and free calponins suggest that calponin is a flexible molecule, and the modifications of serine-175 may also determine the structural flexibility to increase the epitope accessibility. These results provide novel information concerning the structure-function relationships of calponin and its regulation by phosphorylation.
Indexed as
Identifiers
10947974PMC1221287W2065036954What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.