ArticleProceedings of the National Academy of Sciences of the United States of America2000
Ca2+-dependent regulation of synaptic SNARE complex assembly via a calmodulin- and phospholipid-binding domain of synaptobrevin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 107 citations in OpenAlex.
- CaScience advances · 2025Article
- Article
- Synaptobrevin-2 dependent regulation of single synaptic vesicle endocytosis.Molecular biology of the cell · 2021Article
- The Role of Calmodulin vs. Synaptotagmin in Exocytosis.Frontiers in molecular neuroscience · 2021Review
- Structural Roles for the Juxtamembrane Linker Region and Transmembrane Region of Synaptobrevin 2 in Membrane Fusion.Frontiers in cell and developmental biology · 2020Article
- Retrograde trafficking and quality control of yeast synaptobrevin, Snc1, are conferred by its transmembrane domain.Molecular biology of the cell · 2019Article
- Novel Cellular Functions of Very Long Chain-Fatty Acids: Insight From ELOVL4 Mutations.Frontiers in cellular neuroscience · 2019Review
- v-SNARE function in chromaffin cells.Pflugers Archiv : European journal of physiology · 2018Review
- Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion.The Journal of cell biology · 2015Article
- Intracellular Ca2+ signaling and preimplantation development.Advances in experimental medicine and biology · 2015Review
- Membrane-proximal tryptophans of synaptobrevin II stabilize priming of secretory vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin.The Journal of biological chemistry · 2010Article
- Evidence that electrostatic interactions between vesicle-associated membrane protein 2 and acidic phospholipids may modulate the fusion of transport vesicles with the plasma membrane.Molecular biology of the cell · 2009Article
- The mechanisms of multi-component paired-pulse facilitation of neurotransmitter release at the frog neuromuscular junction.Pflugers Archiv : European journal of physiology · 2009Article
- Sphingosine facilitates SNARE complex assembly and activates synaptic vesicle exocytosis.Neuron · 2009Article
- A role for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex dimerization during neurosecretion.Molecular biology of the cell · 2008Article
- Real-time assay for monitoring membrane association of lipid-binding domains.Analytical biochemistry · 2008Article
- Interactions between neuronal fusion proteins explored by molecular dynamics.Biophysical journal · 2008Article
- Productive hemifusion intermediates in fast vesicle fusion driven by neuronal SNAREs.Biophysical journal · 2008Article
- SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic core complex formation is an essential step in exocytosis, and assembly into a superhelical structure may drive synaptic vesicle fusion. To ascertain how Ca(2+) could regulate this process, we examined calmodulin binding to recombinant core complex components. Surface plasmon resonance and pull-down assays revealed Ca(2+)-dependent calmodulin binding (K(d) = 500 nM) to glutathione S-transferase fusion proteins containing synaptobrevin (VAMP 2) domains but not to syntaxin 1 or synaptosomal-associated protein of 25 kDa (SNAP-25). Deletion mutations, tetanus toxin cleavage, and peptide synthesis localized the calmodulin-binding domain to VAMP(77-94), immediately C-terminal to the tetanus toxin cleavage site (Q(76)-F(77)). In isolated synaptic vesicles, Ca(2+)/calmodulin protected native membrane-inserted VAMP from proteolysis by tetanus toxin. Assembly of a (35)S-SNAP-25, syntaxin 1 GST-VAMP(1-96) complex was inhibited by Ca(2+)/calmodulin, but assembly did not mask subsequent accessibility of the calmodulin-binding domain. The same domain contains a predicted phospholipid interaction site. SPR revealed calcium-independent interactions between VAMP(77-94) and liposomes containing phosphatidylserine, which blocked calmodulin binding. Circular dichroism spectroscopy demonstrated that the calmodulin/phospholipid-binding peptide displayed a significant increase in alphahelical content in a hydrophobic environment. These data provide insight into the mechanisms by which Ca(2+) may regulate synaptic core complex assembly and protein interactions with membrane bilayers during exocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.