ArticleProceedings of the National Academy of Sciences of the United States of America2000
Intramolecular control of transcriptional activity by the NK2-specific domain in NK-2 homeodomain proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 100 citations in OpenAlex.
- Differential use of the Nkx2.2 NK2 domain in developing pancreatic islets and neurons.Genes & development · 2023Review
- Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain.Genes & development · 2023Article
- Gene expression and transcriptional regulation driven by transcription factors involved in congenital heart defects.Irish journal of medical science · 2023Article
- A Novel Splicing Mutation c.335-1 G > A in the Cardiac Transcription Factor NKX2-5 Leads to Familial Atrial Septal Defect Through miR-19 and PYK2.Stem cell reviews and reports · 2022Article
- Functional characterization of 5p15.33 risk locus in uveal melanoma reveals rs452384 as a functional variant and NKX2.4 as an allele-specific interactor.American journal of human genetics · 2022Article
- Article
- Nkx2-5 Regulates the Proliferation and Migration of H9c2 Cells.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- The transcription factor NKX2-2 regulates oligodendrocyte differentiation through domain-specific interactions with transcriptional corepressors.The Journal of biological chemistry · 2020Article
- NK homeobox 2.2 functions as tumor suppressor in colorectal cancer due to DNA methylation.Journal of Cancer · 2020Article
- Analysis of NKX2-5 in 439 Chinese Patients with Sporadic Atrial Septal Defect.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Integrator of Stress Responses Calmodulin Binding Transcription Activator 1 (Camta1) Regulates miR-212/miR-132 Expression and Insulin Secretion.The Journal of biological chemistry · 2016Article
- NKX2-5 mutations in an inbred consanguineous population: genetic and phenotypic diversity.Scientific reports · 2015Article
- EWS/FLI utilizes NKX2-2 to repress mesenchymal features of Ewing sarcoma.Genes & cancer · 2015Article
- Arx together with FoxA2, regulates Shh floor plate expression.Developmental biology · 2014Article
- Genome-wide association mapping in dogs enables identification of the homeobox gene, NKX2-8, as a genetic component of neural tube defects in humans.PLoS genetics · 2013Article
- Direct transcriptional regulation of Six6 is controlled by SoxB1 binding to a remote forebrain enhancer.Developmental biology · 2012Article
- Nkx2.2 repressor complex regulates islet β-cell specification and prevents β-to-α-cell reprogramming.Genes & development · 2011Article
- Annelid Distal-less/Dlx duplications reveal varied post-duplication fates.BMC evolutionary biology · 2011Article
- Novel computational analysis of protein binding array data identifies direct targets of Nkx2.2 in the pancreas.BMC bioinformatics · 2011Article
- A de novo mutation in NKX2.5 associated with atrial septal defects, ventricular noncompaction, syncope and sudden death.Clinica chimica acta; international journal of clinical chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The developmentally important homeodomain transcription factors of the NK-2 class contain a highly conserved region, the NK2-specific domain (NK2-SD). The function of this domain, however, remains unknown. The primary structure of the NK2-SD suggests that it might function as an accessory DNA-binding domain or as a protein-protein interaction interface. To assess the possibility that the NK2-SD may contribute to DNA-binding specificity, we used a PCR-based approach to identify a consensus DNA-binding sequences for Nkx2.2, an NK-2 family member involved in pancreas and central nervous system development. The consensus sequence (T(C)(T)AAGT(G)(A)(G)(C)TT) is similar to the known binding sequences for other NK-2 homeodomain proteins, but we show that the NK2-SD does not contribute significantly to specific DNA binding to this sequence. To determine whether the NK2-SD contributes to transactivation, we used GAL4-Nkx2. 2 fusion constructs to map a powerful transcriptional activation domain in the C-terminal region beyond the conserved NK2-SD. Interestingly, this C-terminal region functions as a transcriptional activator only in the absence of an intact NK2-SD. The NK2-SD also can mask transactivation from the paired homeodomain transcription factor Pax6, but it has no effect on transcription by itself. These results demonstrate that the NK2-SD functions as an intramolecular regulator of the C-terminal activation domain in Nkx2.2 and support a model in which interactions through the NK2-SD regulate the ability of NK-2-class proteins to activate specific genes during development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.