ArticleThe Journal of physiology2000
Leptin activation of ATP-sensitive K+ (KATP) channels in rat CRI-G1 insulinoma cells involves disruption of the actin cytoskeleton.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Ion channels and actin: A tale of two friends.Histology and histopathology · 2025Review
- Mechanisms and Functions of Sweet Reception in Oral and Extraoral Organs.International journal of molecular sciences · 2024Review
- The Role of Cdc42 in the Insulin and Leptin Pathways Contributing to the Development of Age-Related Obesity.Nutrients · 2023Review
- The Interplay of Adipokines and Pancreatic Beta Cells in Metabolic Regulation and Diabetes.Biomedicines · 2023Review
- Subcellular trafficking and endocytic recycling of KAmerican journal of physiology. Cell physiology · 2022Review
- Phosphatidylinositol-3 kinase mediates the sweet suppressive effect of leptin in mouse taste cells.Journal of neurochemistry · 2021Article
- What role do fat cells play in pancreatic tissue?Molecular metabolism · 2019Review
- Leptin-induced Trafficking of KInternational journal of molecular sciences · 2019Review
- Stromal Interaction Molecule 1 (STIM1) Regulates ATP-sensitive Potassium (The Journal of biological chemistry · 2017Article
- Leptin-mediated ion channel regulation: PI3K pathways, physiological role, and therapeutic potential.Channels (Austin, Tex.) · 2016Review
- High-resolution micromechanical measurement in real time of forces exerted by living cells.Cell adhesion & migration · 2016Article
- Concerted Trafficking Regulation of Kv2.1 and KATP Channels by Leptin in Pancreatic β-Cells.The Journal of biological chemistry · 2015Article
- Dual action of leptin on rest-firing and stimulated catecholamine release via phosphoinositide 3-kinase-driven BK channel up-regulation in mouse chromaffin cells.The Journal of physiology · 2015Article
- Leptin regulates KATP channel trafficking in pancreatic β-cells by a signaling mechanism involving AMP-activated protein kinase (AMPK) and cAMP-dependent protein kinase (PKA).The Journal of biological chemistry · 2013Article
- Enhanced GLP-1- and sulfonylurea-induced insulin secretion in islets lacking leptin signaling.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Human K(ATP) channelopathies: diseases of metabolic homeostasis.Pflugers Archiv : European journal of physiology · 2010Review
- Article
- Impaired insulin exocytosis in neural cell adhesion molecule-/- mice due to defective reorganization of the submembrane F-actin network.Endocrinology · 2009Article
- Leptin-dependent phosphorylation of PTEN mediates actin restructuring and activation of ATP-sensitive K+ channels.The Journal of biological chemistry · 2009Article
- Leptin regulation of neuronal excitability and cognitive function.Current opinion in pharmacology · 2007Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The role of the cytoskeleton in leptin-induced activation of ATP-sensitive K+ (KATP) channels was examined in rat CRI-G1 insulin-secreting cells using patch clamp and fluorescence imaging techniques. 2. In whole cell recordings, dialysis with the actin filament stabiliser phalloidin (10 microM) prevented KATP channel activation by leptin. 3. Application of the actin filament destabilising agents deoxyribonuclease type 1 (DNase 1; 50 microg ml-1) or cytochalasin B (10 microM) to intact cells or inside-out membrane patches also increased KATP channel activity in a phalloidin-dependent manner. 4. The anti-microtubule agents nocodazole (10 microM) and colchicine (100 microM) had no effect on KATP channel activity. 5. Fluorescence staining of the cells with rhodamine-conjugated phalloidin revealed rapid disassembly of actin filaments by cytochalasin B and leptin, the latter action being prevented by the phosphoinositide 3 (PI 3)-kinase inhibitor LY 294002. 6. Activation of KATP channels by the PI 3-kinase product phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) was also prevented by phalloidin. This is consistent with the notion that leptin activates KATP channels in these cells by an increase in PtdIns(3,4,5)P3 or a similar 3-phosphorylated phosphoinositol lipid, resulting in actin filament disruption.
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