ArticleThe EMBO journal2000
An intact SH3 domain is required for myosin I-induced actin polymerization.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Genome-Wide Association Reveals Signalling-Linked Infection Tolerance in Hibernating Bats.Pathogens (Basel, Switzerland) · 2026Article
- Force-insensitive myosin-I enhances endocytosis robustness through actin network-scale collective ratcheting.Molecular biology of the cell · 2025Article
- Myosin-based nucleation of actin filaments contributes to stereocilia development critical for hearing.Nature communications · 2025Article
- Dissection of the role of a Src homology 3 domain in the evolution of binding preference of paralogous proteins.Genetics · 2024Article
- Myosin-I's motor and actin assembly activation activities are modular and separable in budding yeast clathrin-mediated endocytosis.microPublication biology · 2024Article
- Article
- Review
- Coupled sterol synthesis and transport machineries at ER-endocytic contact sites.The Journal of cell biology · 2021Article
- Article
- Type I myosins anchor actin assembly to the plasma membrane during clathrin-mediated endocytosis.The Journal of cell biology · 2019Article
- Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes.Molecular biology of the cell · 2019Article
- The Sla1 adaptor-clathrin interaction regulates coat formation and progression of endocytosis.Traffic (Copenhagen, Denmark) · 2018Article
- Article
- An Engineered Minimal WASP-Myosin Fusion Protein Reveals Essential Functions for Endocytosis.Developmental cell · 2015Article
- Evolution of the SH3 Domain Specificity Landscape in Yeasts.PloS one · 2015Article
- Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis.PloS one · 2013Article
- Ultrastructural dynamics of proteins involved in endocytic budding.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis.Molecular biology of the cell · 2012Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Bridging membrane and cytoskeleton dynamics in the secretory and endocytic pathways.Nature cell biology · 2011Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast type I myosins (MYO3 and MYO5) are involved in endocytosis and in the polarization of the actin cytoskeleton. The tail of these proteins contains a Tail Homology 2 (TH2) domain that constitutes a putative actin-binding site. Because of the important mechanistic implications of a second ATP-independent actin-binding site, we analyzed its functional relevance in vivo. Even though the myosin tail interacts with actin, and this interaction seems functionally important, deletion of a major portion of the TH2 domain did not abolish interaction. In contrast, we found that the SH3 domain of Myo5p significantly contributes to this interaction, implicating other proteins. We found that Vrp1p, the yeast homolog of WIP [Wiskott-Aldrich syndrome protein (WASP)-interacting protein], seems necessary to sustain the Myo5p tail-F-actin interaction. Consistent with recent results implicating the yeast type I myosins in regulating actin polymerization in vivo, we demonstrate that the C-terminal domain of Myo5p is able to induce cytosol-dependent actin polymerization in vitro, and that this activity requires both an intact Myo5p SH3 domain and Vrp1p.
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