ArticleRNA (New York, N.Y.)2000
Mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rRNA affect the function of the decoding center of the ribosome.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Architectural principles of the ribosomal large subunit revealed by A-helix spatial organization.Scientific reports · 2026Article
- Varying strength of selection contributes to the intragenomic diversity of rRNA genes.Nature communications · 2022Article
- Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation.Non-coding RNA research · 2022Article
- Experimental Confirmation that an UncommonAntimicrobial agents and chemotherapy · 2022Article
- Poly(A)-Binding Protein Regulates the Efficiency of Translation Termination.Cell reports · 2020Article
- Age-Dependent and Tissue-Specific Alterations in the rDNA Clusters of theBiomolecules · 2020Article
- Levels of sdRNAs in cytoplasm and their association with ribosomes are dependent upon stress conditions but independent from snoRNA expression.Scientific reports · 2019Article
- Maturation of the 90S pre-ribosome requires Mrd1 dependent U3 snoRNA and 35S pre-rRNA structural rearrangements.Nucleic acids research · 2018Article
- tRNA-derived short RNAs bind to Saccharomyces cerevisiae ribosomes in a stress-dependent manner and inhibit protein synthesis in vitro.FEMS yeast research · 2016Article
- Intersubunit Bridges of the Bacterial Ribosome.Journal of molecular biology · 2016Review
- Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.Nature communications · 2015Article
- Review
- Random mutagenesis of yeast 25S rRNA identify bases critical for 60S subunit structural integrity and function.Translation (Austin, Tex.) · 2013Article
- Yeast 18 S rRNA is directly involved in the ribosomal response to stringent AUG selection during translation initiation.The Journal of biological chemistry · 2010Article
- Missense suppressor mutations in 16S rRNA reveal the importance of helices h8 and h14 in aminoacyl-tRNA selection.RNA (New York, N.Y.) · 2010Article
- Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1.Nucleic acids research · 2009Article
- Accessibility of 18S rRNA in human 40S subunits and 80S ribosomes at physiological magnesium ion concentrations--implications for the study of ribosome dynamics.RNA (New York, N.Y.) · 2005Article
- A functional relationship between helix 1 and the 900 tetraloop of 16S ribosomal RNA within the bacterial ribosome.RNA (New York, N.Y.) · 2005Article
- Leaky termination at premature stop codons antagonizes nonsense-mediated mRNA decay in S. cerevisiae.RNA (New York, N.Y.) · 2004Article
- Genetic evidence against the 16S ribosomal RNA helix 27 conformational switch model.RNA (New York, N.Y.) · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A dynamic structural rearrangement in the phylogenetically conserved helix 27 of Escherichia coli 16S rRNA has been proposed to directly affect the accuracy of translational decoding by switching between "accurate" and "error-prone" conformations. To examine the function of helix 27 in eukaryotes, random and site-specific mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rRNA have been characterized. Mutations at positions of yeast 18S rRNA corresponding to E. coli 886 (rdn8), 888 (rdn6), and 912 (rdn4) increased translational accuracy in vivo and in vitro, and caused a reduction in tRNA binding to the A-site of mutant ribosomes. The double rdn4rdn6 mutation separated the killing and stop-codon readthrough effects of the aminoglycoside antibiotic, paromomycin, implicating a direct involvement of yeast helix 27 in accurate recognition of codons by tRNA or release factor eRF1. Although our data in yeast does not support a conformational switch model analogous to that proposed for helix 27 of E. coli 16S rRNA, it strongly suggests a functional conservation of this region in tRNA selection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.