ArticleRNA (New York, N.Y.)2000
Mutational study reveals that tertiary interactions are conserved in ribosomal frameshifting pseudoknots of two luteoviruses.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Ensemble simulations: folding, unfolding and misfolding of a high-efficiency frameshifting RNA pseudoknot.Nucleic acids research · 2017Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- RNASTAR: an RNA STructural Alignment Repository that provides insight into the evolution of natural and artificial RNAs.RNA (New York, N.Y.) · 2012Article
- Annexin A2 binds RNA and reduces the frameshifting efficiency of infectious bronchitis virus.PloS one · 2011Article
- An intermolecular RNA triplex provides insight into structural determinants for the pseudoknot stimulator of -1 ribosomal frameshifting.Nucleic acids research · 2010Article
- Stem-loop structure of Cocksfoot mottle virus RNA is indispensable for programmed -1 ribosomal frameshifting.Virus research · 2009Article
- Footprinting analysis of BWYV pseudoknot-ribosome complexes.RNA (New York, N.Y.) · 2009Article
- Frameshifting RNA pseudoknots: structure and mechanism.Virus research · 2009Review
- The stimulatory RNA of the Visna-Maedi retrovirus ribosomal frameshifting signal is an unusual pseudoknot with an interstem element.RNA (New York, N.Y.) · 2008Article
- The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical.RNA (New York, N.Y.) · 2006Article
- Pairwise coupling analysis of helical junction hydrogen bonding interactions in luteoviral RNA pseudoknots.Biochemistry · 2006Article
- Dissecting non-canonical interactions in frameshift-stimulating mRNA pseudoknots.Journal of biomolecular NMR · 2006Article
- Translational control in positive strand RNA plant viruses.Virology · 2006Review
- A loop 2 cytidine-stem 1 minor groove interaction as a positive determinant for pseudoknot-stimulated -1 ribosomal frameshifting.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Crystal structure of a luteoviral RNA pseudoknot and model for a minimal ribosomal frameshifting motif.Biochemistry · 2005Article
- Comparative mutational analysis of cis-acting RNA signals for translational frameshifting in HIV-1 and HTLV-2.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the putative replicase of potato leafroll virus (PLRV) is regulated by -1 ribosomal frameshifting in which a primary viral transcript has two overlapping open reading frames (ORFs). A region of 39 nt at the junction of the two ORFs is essential for frameshifting to occur. It has been shown to harbor two signals, one active on the level of the primary structure, termed the slippery sequence, and one component that forms a secondary or tertiary level structure, described as either a pseudoknot or a stem-loop motif. We have performed extensive site-directed mutagenesis of the frameshifting region and analyzed individual mutants for their ability to promote -1 frameshifting in vitro. Detailed comparison of our results with analogous mutants in the frameshifting region of the evolutionarily related beet western yellow virus, for which a crystal structure is available, unequivocally argues for the pseudoknot to be the structural motif necessary for the frameshifting function in PLRV transcripts. Mutations in PLRV that affect putative pseudoknot-specific tertiary-base interactions drastically affect frameshifting activity. In addition, a specific deletion mutant was identified that displayed PLRV wild-type frameshifting activity with only 22 nt available for pseudoknot formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.