ArticleEuropean journal of immunology2000
Structure-function analysis of a lupus anti-DNA autoantibody: central role of the heavy chain complementarity-determining region 3 Arg in binding of double- and single-stranded DNA.
Article in European journal of immunology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Physicochemical convergence in antibody CDR3-VH repertoires recognizing phosphorothioate-modified oligonucleotides backbone.Frontiers in immunology · 2026Article
- Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins.Communications biology · 2024Article
- A Human MSH6 Germline Variant Associated With Systemic Lupus Erythematosus Induces Lupus-like Disease in Mice.ACR open rheumatology · 2022Article
- Heavy chain-only antibody genes in fish evolved to generate unique CDR3 repertoire.European journal of immunology · 2022Article
- Autoantibody-dependent amplification of inflammation in SLE.Cell death & disease · 2020Article
- Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used.Frontiers in immunology · 2018Article
- Anti-DNA antibodies--quintessential biomarkers of SLE.Nature reviews. Rheumatology · 2016Review
- Potential serum and urine biomarkers in patients with lupus nephritis and the unsolved problems.Open access rheumatology : research and reviews · 2016Review
- Functional Versatility of AGY Serine Codons in Immunoglobulin Variable Region Genes.Frontiers in immunology · 2016Article
- An entity evolving into a community: defining the common ancestor and evolutionary trajectory of chronic lymphocytic leukemia stereotyped subset #4.Molecular medicine (Cambridge, Mass.) · 2015Article
- Predominant role for activation-induced cytidine deaminase in generating IgG anti-nucleosomal antibodies of murine SLE.Journal of autoimmunity · 2015Article
- Temporal dynamics of clonal evolution in chronic lymphocytic leukemia with stereotyped IGHV4-34/IGKV2-30 antigen receptors: longitudinal immunogenetic evidence.Molecular medicine (Cambridge, Mass.) · 2013Article
- Somatic mutagenesis in autoimmunity.Autoimmunity · 2013Review
- Linking complement and anti-dsDNA antibodies in the pathogenesis of systemic lupus erythematosus.Immunologic research · 2013Review
- Activation-induced deaminase contributes to the antibody-independent role of B cells in the development of autoimmunity.Autoimmunity · 2012Article
- Specific cross-reaction of anti-dsDNA antibody with platelet integrin GPIIIa49-66.Autoimmunity · 2010Article
- Somatic hypermutation as a generator of antinuclear antibodies in a murine model of systemic autoimmunity.The Journal of experimental medicine · 2010Article
- Pathogenic and Epiphenomenal Anti-DNA Antibodies in SLE.Autoimmune diseases · 2010Article
- Article
- Nature and functions of autoantibodies.Nature clinical practice. Rheumatology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
To determine the contribution of the somatic point mutations and that of the complementarity-determining region (CDR)3 Arg to DNA binding, we engineered the germline V(H) and V(kappa) gene revertant and site-mutagenized the CDR3 Arg residues of the mutated and "antigen-selected" mAb 412.67. This anti-DNA autoantibody was derived from B-1 cells of a lupus patient and bore two H-CDR3 Arg, Arg105 and Arg107, encoded by N segment additions, and one kappa-CDR3 Arg, Arg97, resulting from a point mutation (Kasaian et al. 1994. J. Immunol. 152: 3137-3151; Kasaian et al. 1995. Ann. N.Y Acad. Sci. 764: 410-423). The germ-line revertant bound double-stranded (ds) DNA and single-stranded (ss) DNA as effectively as its wild-type counterpart (relative avidity: 6.4x10(-7) and 9.9x10(-9) vs. 6.7x10(-7) and 9.1 x10(-9) g/microl), raising the possibility that an antigen other than DNA was responsible for the selection of the mAb 412.67 V(H) and V(kappa) point mutations. H-CDR3 Arg105 and Arg107 were both required for dsDNA binding, but either Arg105 or Arg107 was sufficient for ssDNA binding. The central role of Arg105 and Arg107 in DNA binding reflected their solvent-exposed orientation at the apex of the H-CDR3 main loop. Consistent with its inward orientation afar from the antigen-binding surface, the kappa-CDR3 Arg97 played no role in either dsDNA or ssDNA binding.
Indexed as
Identifiers
10940891PMC4623572W1983263967What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.