ArticleJournal of bacteriology2000
A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
123 citing papers in PubMed, 236 citations in OpenAlex.
- A previously uncharacterized Fur-family metalloregulator integrates iron- and manganese-sensing to control virulence gene regulatory networks in Brucella.Scientific reports · 2025Article
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- Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review.Frontiers in cellular and infection microbiology · 2025Review
- Copper sensing transcription factor ArsR2 regulates VjbR to sustain virulence inEmerging microbes & infections · 2024Article
- A Review on the Methodology and Use of the Pregnant Mouse Model in the Study ofMicroorganisms · 2024Review
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- Small Molecule Inhibitors against the Bacterial PathogenCurrent medicinal chemistry · 2024Review
- TheMicroorganisms · 2023Article
- Antibiotic persistence of intracellular Brucella abortus.PLoS neglected tropical diseases · 2022Article
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- Nucleomodulin BspJ as an effector promotes the colonization ofJournal of veterinary science · 2022Article
- The VirB System Plays a Crucial Role inInternational journal of molecular sciences · 2021Review
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Different Impacts of MucR Binding to theBiomolecules · 2020Article
- Article
- Proteomics of Brucella.Proteomes · 2020Review
- Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in themBio · 2020Article
- Article
- A Carbonic Anhydrase Pseudogene Sensitizes SelectJournal of bacteriology · 2019Article
- PhiA, a Peptidoglycan Hydrolase Inhibitor ofInfection and immunity · 2019Article
63 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As part of a Brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic DNA fragment containing a locus (virB) highly homologous to bacterial type IV secretion systems. The B. abortus virB locus is a collinear arrangement of 13 open reading frames (ORFs). Between virB1 and virB2 and downstream of ORF12, two degenerated, palindromic repeat sequences characteristic of Brucella intergenic regions were found. Gene reporter studies demonstrated that the B. abortus virB locus constitutes an operon transcribed from virB1 which is turned on during the stationary phase of growth. A B. abortus polar virB1 mutant failed to replicate in HeLa cells, indicating that the virB operon plays a critical role in intracellular multiplication. Mutants with polar and nonpolar mutations introduced in virB10 showed different behaviors in mice and in the HeLa cell infection assay, suggesting that virB10 per se is necessary for the correct function of this type IV secretion apparatus. Mouse infection assays demonstrated that the virB operon constitutes a major determinant of B. abortus virulence. It is suggested that putative effector molecules secreted by this type IV secretion system determine routing of B. abortus to an endoplasmic reticulum-related replication compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.