ArticlePlant physiology2000
A minimal serine/threonine protein kinase circadianly regulates phosphoenolpyruvate carboxylase activity in crassulacean acid metabolism-induced leaves of the common ice plant.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 118 citations in OpenAlex.
- Facultative CAM and photosynthetic electron transport: unravelling the salinity acclimation puzzle inFrontiers in plant science · 2026Review
- Cryptic CAM photosynthesis in Joshua tree (Yucca brevifolia, Y. jaegeriana).The New phytologist · 2025Article
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- Diel dynamics of multi-omics in elkhorn fern provide new insights into weak CAM photosynthesis.Plant communications · 2023Article
- Genome-wide mapping of DNase I hypersensitive sites in pineapple leaves.Frontiers in genetics · 2023Article
- Differential timing of gene expression and recruitment in independent origins of CAM in the Agavoideae (Asparagaceae).The New phytologist · 2022Article
- Article
- Shared expression of crassulacean acid metabolism (CAM) genes pre-dates the origin of CAM in the genus Yucca.Journal of experimental botany · 2019Article
- Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare.Journal of experimental botany · 2019Article
- Hierarchical clustering reveals unique features in the diel dynamics of metabolites in the CAM orchid Phalaenopsis.Journal of experimental botany · 2019Article
- Laying the Foundation for Crassulacean Acid Metabolism (CAM) Biodesign: Expression of the CFrontiers in plant science · 2019Article
- CO2 and O2 dynamics in leaves of aquatic plants with C3 or CAM photosynthesis - application of a novel CO2 microsensor.Annals of botany · 2018Article
- Altered Gene Regulatory Networks Are Associated With the Transition From CFrontiers in plant science · 2018Article
- Expression Profiles of Phosphoenolpyruvate Carboxylase and Phosphoenolpyruvate Carboxylase Kinase Genes inFrontiers in plant science · 2018Article
- The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.Nature communications · 2017Article
- Phosphorylation of PhosphoThe Plant cell · 2017Article
- Quantitative in vivo phosphoproteomics reveals reversible signaling processes during nitrogen starvation and recovery in the biofuel model organismBiotechnology for biofuels · 2017Article
- Article
- Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.Plant physiology · 2016Article
- Evolution of the Phosphoenolpyruvate Carboxylase Protein Kinase Family in C3 and C4 Flaveria spp.Plant physiology · 2014Article
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant phosphoenolpyruvate carboxylase (PEPc) activity and allosteric properties are regulated by PEPc kinase (PPcK) through reversible phosphorylation of a specific serine (Ser) residue near the N terminus. We report the molecular cloning of PPcK from the facultative Crassulacean acid metabolism (CAM) common ice plant (Mesembryanthemum crystallinum), using a protein-kinase-targeted differential display reverse transcriptase-polymerase chain reaction approach. M. crystallinum PPcK encodes a minimal, Ca(2+)-independent Ser/threonine protein kinase that is most closely related to calcium-dependent protein kinases, yet lacks both the calmodulin-like and auto-inhibitory domains typical of plant calcium-dependent protein kinase. In the common ice plant PPcK belongs to a small gene family containing two members. McPPcK transcript accumulation is controlled by a circadian oscillator in a light-dependent manner. McPPcK encodes a 31.8-kD polypeptide (279 amino acids), making it among the smallest protein kinases characterized to date. Initial biochemical analysis of the purified, recombinant McPPcK gene product documented that this protein kinase specifically phosphorylates PEPc from CAM and C(4) species at a single, N-terminal Ser (threonine) residue but fails to phosphorylate mutated forms of C(4) PEPc in which this specific site has been changed to tyrosine or aspartate. McPPcK activity was specific for PEPc, Ca(2+)-insensitive, and displayed an alkaline pH optimum. Furthermore, recombinant McPPcK was shown to reverse the sensitivity of PEPc activity to L-malate inhibition in CAM-leaf extracts prepared during the day, but not at night, documenting that PPcK contributes to the circadian regulation of photosynthetic carbon flux in CAM plants.
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