ArticlePlant physiology2000
Calcium-mediated signaling during sandalwood somatic embryogenesis. Role for exogenous calcium as second messenger.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 86 citations in OpenAlex.
- An improved and simplified somatic embryogenesis protocol in Chir pine (Pinus roxburghii).Scientific reports · 2025Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Calcium Dynamics, WUSCHEL Expression and Callose Deposition during Somatic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
- Calcium dynamics and modulation in carrot somatic embryogenesis.Frontiers in plant science · 2023Article
- Investigation of Roles ofPlants (Basel, Switzerland) · 2022Article
- Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA).Scientific reports · 2020Article
- Review
- Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation.International journal of molecular sciences · 2019Article
- Addition of ionophore A23187 increases the efficiency of3 Biotech · 2018Article
- Involvement of Calcium and Calmodulin in Nitric Oxide-Regulated Senescence of Cut Lily Flowers.Frontiers in plant science · 2018Article
- Differential proteome analysis during early somatic embryogenesis in Musa spp. AAA cv. Grand Naine.Plant cell reports · 2017Article
- The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families inFEBS open bio · 2017Article
- Article
- De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing.Protoplasma · 2016Article
- Review
- Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture.Frontiers in plant science · 2016Article
- Transcriptome profiling and in silico analysis of somatic embryos in Japanese larch (Larix leptolepis).Plant cell reports · 2012Article
- Mechanisms of xylanase-induced nitric oxide and phosphatidic acid production in tomato cells.Planta · 2011Article
- Molecular regulation of somatic embryogenesis in potato: an auxin led perspective.Plant molecular biology · 2008Article
- Characterization of three somatic embryogenesis receptor kinase genes from wheat, Triticum aestivum.Plant cell reports · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possible involvement of Ca(2+)-mediated signaling in the induction/regulation of somatic embryogenesis from pro-embryogenic cells of sandalwood (Santalum album) has been investigated. (45)Ca(2+)-uptake studies and fura-2 fluorescence ratio photometry were used to measure changes in [Ca(2+)](cyt) of pro-embryogenic cells in response to culture conditions conducive for embryo development. Sandalwood pro-embryogenic cell masses (PEMs) are obtained in the callus proliferation medium that contains the auxin 2,4-dichlorophenoxyacetic acid. Subculture of PEMs into the embryo differentiation medium, which lacks 2,4-dichlorophenoxyacetic acid and has higher osmoticum, results in a 4-fold higher (45)Ca(2+) incorporation into the symplast. Fura-2 ratiometric analysis corroboratively shows a 10- to 16-fold increase in the [Ca(2+)](cyt) of PEMs, increasing from a resting concentration of 30 to 50 nM to 650 to 800 nM. Chelation of exogenous Ca(2+) with ethyleneglycol-bis(aminoethyl ether)-N,N'-tetraacetic acid arrests such an elevation in [Ca(2+)](cyt). Exogenous Ca(2+) when chelated or deprived also arrests embryo development and inhibits the accumulation of a sandalwood Ca(2+)-dependent protein kinase. However, such culture conditions do not cause cell death as the PEMs continue to proliferate to form larger cell clumps. Culture treatment with N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide reduced embryogenic frequency by 85%, indicating that blockage of Ca(2+)-mediated signaling pathway(s) involving sandalwood Ca(2+)-dependent protein kinase and/or calmodulin causes the inhibition of embryogenesis. The observations presented are evidence to suggest a second messenger role for exogenous Ca(2+) during sandalwood somatic embryogenesis.
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