ArticlePlant physiology2000
A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 206 citations in OpenAlex.
- Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.Plant physiology · 2026Article
- The initiation of embryo growth in imbibed celery mericarps marks a key mechanism by which temperature signals are integrated to regulate germination timing.Journal of experimental botany · 2026Article
- The eTM-miR3699-MAN7 mediated cell wall degradation in regulating embryogenic cell formation during the early stage of somatic embryogenesis in apple.Horticulture research · 2026Article
- Anatomical limitations in adventitious root formation revealed by magnetic resonance imaging, infrared spectroscopy, and histology of rose genotypes with contrasting rooting phenotypes.Journal of experimental botany · 2024Article
- Identification of Reference Genes for Precise Expression Analysis during Germination inInternational journal of molecular sciences · 2023Article
- Effect of Reactive Oxygen Scavenger N,N'-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize.International journal of molecular sciences · 2023Article
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- Tomato ABA-IMPORTING TRANSPORTER 1.1 inhibits seed germination under high salinity conditions.Plant physiology · 2023Article
- Heterologous expression ofFrontiers in plant science · 2023Article
- Functional variants ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
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- A Possible Role of the Aleurone Expressed GeneFrontiers in plant science · 2019Article
- Carbohydrate reserves and seed development: an overview.Plant reproduction · 2018Review
- Mannans and endo-β-mannanase transcripts are located in different seed compartments during Brassicaceae germination.Planta · 2018Article
- Reactive Oxygen Species Generated by NADPH Oxidases Promote Radicle Protrusion and Root Elongation during Rice Seed Germination.International journal of molecular sciences · 2017Article
- Seed Biology Updates - Highlights and New Discoveries in Seed Dormancy and Germination Research.Frontiers in plant science · 2017Review
- α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Plant hormone-mediated regulation of stress responses.BMC plant biology · 2016Review
- Genetic Variation for Thermotolerance in Lettuce Seed Germination Is Associated with Temperature-Sensitive Regulation of ETHYLENE RESPONSE FACTOR1 (ERF1).Plant physiology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endo-beta-mannanase (EC 3.2.1.78) is involved in hydrolysis of the mannan-rich cell walls of the tomato (Lycopersicon esculentum Mill.) endosperm during germination and post-germinative seedling growth. Different electrophoretic isoforms of endo-beta-mannanase are expressed sequentially in different parts of the endosperm, initially in the micropylar endosperm cap covering the radicle tip and subsequently in the remaining lateral endosperm surrounding the rest of the embryo. We have isolated a cDNA from imbibed tomato seeds (LeMAN2) that shares 77% deduced amino acid sequence similarity with a post-germinative tomato mannanase (LeMAN1). When expressed in Escherichia coli, the protein encoded by LeMAN2 cDNA was recognized by anti-mannanase antibody and exhibited endo-beta-mannanase activity, confirming the identity of the gene. LeMAN2 was expressed exclusively in the endosperm cap tissue of tomato seeds prior to radicle emergence, whereas LeMAN1 was expressed only in the lateral endosperm after radicle emergence. LeMAN2 mRNA accumulation and mannanase activity were induced by gibberellin in gibberellin-deficient gib-1 mutant seeds but were not inhibited by abscisic acid in wild-type seeds. Distinct mannanases are involved in germination and post-germinative growth, with LeMAN2 being associated with endosperm cap weakening prior to radicle emergence, whereas LeMAN1 mobilizes galactomannan reserves in the lateral endosperm.
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