ArticleMolecular and cellular biology2000
Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 69 citations in OpenAlex.
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- Entirely enzymatic nanofabrication of DNA-protein conjugates.Nucleic acids research · 2017Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Epigenetic control of Agrobacterium T-DNA integration.Biochimica et biophysica acta · 2011Review
- QTLs controlling the production of transgenic and adventitious roots in Brassica oleracea following treatment with Agrobacterium rhizogenes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells.EMBO reports · 2004Article
- T-DNA integration in Arabidopsis chromosomes. Presence and origin of filler DNA sequences.Plant physiology · 2003Article
- Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates.Plant physiology · 2003Article
- Plant transformation by coinoculation with a disarmed Agrobacterium tumefaciens strain and an Escherichia coli strain carrying mobilizable transgenes.Applied and environmental microbiology · 2003Article
- Transgene structures in T-DNA-inserted rice plants.Plant molecular biology · 2003Article
- Excision of selectable marker genes from transgenic plants.Nature biotechnology · 2002Review
- Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration.The EMBO journal · 2001Article
- Plant gene expression response to Agrobacterium tumefaciens.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- An Agrobacterium VirE2 channel for transferred-DNA transport into plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens, a gram-negative soil bacterium, transfers DNA to many plant species. In the plant cell, the transferred DNA (T-DNA) is integrated into the genome. An in vitro ligation-integration assay has been designed to investigate the mechanism of T-DNA ligation and the factors involved in this process. The VirD2 protein, which is produced in Agrobacterium and is covalently attached to T-DNA, did not, under our assay conditions, ligate T-DNA to a model target sequence in vitro. We tested whether plant extracts could ligate T-DNA to target oligonucleotides in our test system. The in vitro ligation-integration reaction did indeed take place in the presence of plant extracts. This reaction was inhibited by dTTP, indicating involvement of a plant DNA ligase. We found that prokaryotic DNA ligases could substitute for plant extracts in this reaction. Ligation of the VirD2-bound oligonucleotide to the target sequence mediated by T4 DNA ligase was less efficient than ligation of a free oligonucleotide to the target. T-DNA ligation mediated by a plant enzyme(s) or T4 DNA ligase requires ATP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.