ArticleMolecular and cellular biology2000
Termination and peptide release at the upstream open reading frame are required for downstream translation on synthetic shunt-competent mRNA leaders.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Upstream ORFs are prevalent translational repressors in vertebrates.The EMBO journal · 2016Article
- Non-canonical translation in RNA viruses.The Journal of general virology · 2012Review
- Initiation of translation of the FMR1 mRNA Occurs predominantly through 5'-end-dependent ribosomal scanning.Journal of molecular biology · 2011Article
- Translational bypassing without peptidyl-tRNA anticodon scanning of coding gap mRNA.The EMBO journal · 2008Article
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- Translation of cIAP2 mRNA is mediated exclusively by a stress-modulated ribosome shunt.Molecular and cellular biology · 2008Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- Tethering of eIF4G to adenoviral mRNAs by viral 100k protein drives ribosome shunting.Genes & development · 2004Article
- 5'-untranslated regions with multiple upstream AUG codons can support low-level translation via leaky scanning and reinitiation.Nucleic acids research · 2004Article
- An upstream open reading frame impedes translation of the huntingtin gene.Nucleic acids research · 2002Article
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- New ways of initiating translation in eukaryotes?Molecular and cellular biology · 2001Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown recently that a stable hairpin preceded by a short upstream open reading frame (uORF) promotes nonlinear ribosome migration or ribosome shunt on a synthetic mRNA leader (M. Hemmings-Mieszczak and T. Hohn, RNA 5:1149-1157, 1999). We have now used the model mRNA leader to study further the mechanism of shunting in vivo and in vitro. We show that a full cycle of translation of the uORF, including initiation, elongation, and termination, is a precondition for the ribosome shunt across the stem structure to initiate translation downstream. Specifically, AUG recognition and the proper release of the nascent peptide are necessary and sufficient for shunting. Furthermore, the stop codon context must not impede downstream reinitiation. Translation of the main ORF was inhibited by replacement of the uORF by coding sequences repressing reinitiation but stimulated by the presence of the virus-specific translational transactivator of reinitiation (cauliflower mosaic virus pVI). Our results indicate reinitiation as the mechanism of translation initiation on the synthetic shunt-competent mRNA leader and suggest that uORF-dependent shunting is more prevalent than previously anticipated. Within the above constraints, uORF-dependent shunting is quite tolerant of uORF and stem sequences and operates in systems as diverse as plants and fungi.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.