ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2000
Urease activity may contribute to the ability of Actinobacillus pleuropneumoniae to establish infection.
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Comparative Efficacy in Challenge Dose Models of a Toxin Expressing Whole-Cell Vaccine against Eight Serovars ofAnimals : an open access journal from MDPI · 2022Article
- Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
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- Expression of urease by Haemophilus influenzae during human respiratory tract infection and role in survival in an acid environment.BMC microbiology · 2011Article
- Proteomic expression profiling of Haemophilus influenzae grown in pooled human sputum from adults with chronic obstructive pulmonary disease reveal antioxidant and stress responses.BMC microbiology · 2010Article
- Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae.Journal of molecular biology · 2009Article
- Genome degradation in Brucella ovis corresponds with narrowing of its host range and tissue tropism.PloS one · 2009Article
- Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium.Infection and immunity · 2007Article
- Two TonB systems in Actinobacillus pleuropneumoniae: their roles in iron acquisition and virulence.Infection and immunity · 2004Article
- Nontypeable Haemophilus influenzae gene expression induced in vivo in a chinchilla model of otitis media.Infection and immunity · 2003Article
- Novel genes affecting urease acivity in Actinobacillus pleuropneumoniae.Journal of bacteriology · 2001Article
- Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response.Infection and immunity · 2001Article
- Virulence factors of Actinobacillus pleuropneumoniae involved in colonization, persistence and induction of lesions in its porcine host.Veterinary researchReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The contribution of urease activity to the pathogenesis of Actinobacillus pleuropneumoniae was investigated using 2 different urease-negative transposon mutants of the virulent serotype 1 strain, CM5 Nalr. One mutant, cbiK::Tn10, is deficient in the uptake of nickel, a cofactor required for urease activity. The other mutant, ureG::Tn10, is unable to produce active urease due to mutation of the urease accessory gene, ureG. In aerosol challenge experiments, pigs developed acute pleuropneumonia following exposure to high doses (10(6) cfu/mL) of the parental strain, CM5 Nalr, and to the cbiK::Tn10 mutant. When low dose (10(3) cfu/mL) challenges were used, neither urease-negative mutant was able to establish infection, whereas the parental strain was able to colonize and cause lesions consistent with acute pleuropneumonia in 8 of the 20 pigs challenged. These findings suggest that urease activity may be needed for A. pleuropneumoniae to establish infection in the respiratory tract of pigs.
Indexed as
Identifiers
10935879PMC1189605W1910328467What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.