ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
P2X7 receptors in Müller glial cells from the human retina.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 180 citations in OpenAlex.
- Revisiting astrocytic calcium signaling in the brain.Fundamental research · 2024Review
- Intraocular kinetics of pathological ATP after photoreceptor damage in rhegmatogenous retinal detachment.Japanese journal of ophthalmology · 2024Article
- Saffron and retinal neurodegenerative diseases: Relevance of chemical composition.Journal of anatomy · 2023Article
- The Role of Müller Cells in Diabetic Macular Edema.Investigative ophthalmology & visual science · 2023Review
- Emerging roles of the P2X7 receptor in cancer pain.Purinergic signalling · 2023Review
- Elucidating glial responses to products of diabetes-associated systemic dyshomeostasis.Progress in retinal and eye research · 2023Review
- Astrocyte adaptation in Alzheimer's disease: a focus on astrocytic P2X7R.Essays in biochemistry · 2023Review
- P2X7 Is Involved in the Mouse Retinal Degeneration via the Coordinated Actions in Different Retinal Cell Types.Antioxidants (Basel, Switzerland) · 2023Article
- Glaucoma and microglia-induced neuroinflammation.Frontiers in ophthalmology · 2023Review
- Photochemical Restoration of Light Sensitivity in the Degenerated Canine Retina.Pharmaceutics · 2022Article
- Purinergic Receptors P2X7 and P2X4 as Markers of Disease Progression in the rd10 Mouse Model of Inherited Retinal Dystrophy.International journal of molecular sciences · 2022Article
- Inherited Retinal Dystrophies: Role of Oxidative Stress and Inflammation in Their Physiopathology and Therapeutic Implications.Antioxidants (Basel, Switzerland) · 2022Review
- Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines.Translational vision science & technology · 2022Article
- Astroglial and Microglial Purinergic P2X7 Receptor as a Major Contributor to Neuroinflammation during the Course of Multiple Sclerosis.International journal of molecular sciences · 2021Review
- Astrocytic and Oligodendrocytic P2X7 Receptors Determine Neuronal Functions in the CNS.Frontiers in molecular neuroscience · 2021Article
- Retinal Cell Protection in Ocular Excitotoxicity Diseases. Possible Alternatives Offered by Microparticulate Drug Delivery Systems and Future Prospects.Pharmaceutics · 2020Review
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.eLife · 2018Article
- Special Issue for Purinergic Receptors, Particularly P2XVision (Basel, Switzerland) · 2018Article
- Physiology of Astroglia.Physiological reviews · 2018Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP has been shown to be an important extracellular signaling molecule. There are two subgroups of receptors for ATP (and other purines and pyrimidines): the ionotropic P2X and the G-protein-coupled P2Y receptors. Different subtypes of these receptors have been identified by molecular biology, but little is known about their functional properties in the nervous system. Here we present data for the existence of P2 receptors in Müller (glial) cells of the human retina. The cells were studied by immunocytochemistry, electrophysiology, Ca(2+)-microfluorimetry, and molecular biology. They displayed both P2Y and P2X receptors. Freshly enzymatically isolated cells were used throughout the study. Although the [Ca(2+)](i) response to ATP was dominated by release from intracellular stores, there is multiple evidence that the ATP-induced membrane currents were caused by an activation of P2X(7) receptors. Immunocytochemistry and single-cell RT-PCR revealed the expression of P2X(7) receptors by Müller cells. In patch-clamp studies, we found that (1) benzoyl-benzoyl ATP (BzATP) was the most effective agonist to evoke large inward currents and (2) the currents were abolished by P2X antagonists; however, (3) long-lasting application of BzATP did not cause an opening of large pores in addition to the cationic channels. By microfluorimetry it was shown that the P2X receptors mediated a Ca(2+) influx that contributed a small component to the total [Ca(2+)](i) response. Activation of P2X receptors may modulate the uptake of neurotransmitters from the extracellular space by Müller cells in the retina.
Indexed as
Identifiers
10934244PMC6772577W2146908013What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.