ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
A novel leg-shaking Drosophila mutant defective in a voltage-gated K(+)current and hypersensitive to reactive oxygen species.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
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- Dichotomous cis-regulatory motifs mediate the maturation of the neuromuscular junction by retrograde BMP signaling.Nucleic acids research · 2022Article
- Tired and stressed: Examining the need for sleep.The European journal of neuroscience · 2020Review
- A Drosophila Model of Essential Tremor.Scientific reports · 2018Article
- Generation and characterization of new alleles of quiver (qvr) that encodes an extracellular modulator of the Shaker potassium channel.Journal of neurogenetics · 2017Article
- Mutation of orthologous prickle genes causes a similar epilepsy syndrome in flies and humans.Annals of clinical and translational neurology · 2016Article
- Article
- A neuron-glia interaction involving GABA transaminase contributes to sleep loss in sleepless mutants.Molecular psychiatry · 2015Article
- Identification of pharmacological targets in amyotrophic lateral sclerosis through genomic analysis of deregulated genes and pathways.Current genomics · 2012Article
- Drosophila QVR/SSS modulates the activation and C-type inactivation kinetics of Shaker K(+) channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Modulation of the frequency response of Shaker potassium channels by the quiver peptide suggesting a novel extracellular interaction mechanism.Journal of neurogenetics · 2010Article
- Article
- SLEEPLESS, a Ly-6/neurotoxin family member, regulates the levels, localization and activity of Shaker.Nature neuroscience · 2010Article
- Dopamine and aging: intersecting facets.Neurochemical research · 2009Review
- Effects of social isolation on neuromuscular excitability and aggressive behaviors in Drosophila: altered responses by Hk and gsts1, two mutations implicated in redox regulation.Journal of neurogenetics · 2009Article
- Identification of SLEEPLESS, a sleep-promoting factor.Science (New York, N.Y.) · 2008Article
- Social interaction-mediated lifespan extension of Drosophila Cu/Zn superoxide dismutase mutants.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Drosophila Hyperkinetic mutants have reduced sleep and impaired memory.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis.BMC genomics · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
1,1'-Dimethyl-4,4'-bipyridinium dichloride (methyl viologen; paraquat), an herbicide that causes depletion of NADPH and generates excessive reactive oxygen species (ROS) in vivo, has been used to screen for ROS-sensitive Drosophila mutants. One mutant so isolated, named quiver(1) (qvr(1)), has a leg-shaking phenotype. Mutants of the Shaker (Sh), Hyperkinetic (Hk), and ether a go-go (eag) genes, which encode different K(+) channel subunits that regulate the A-type K(+) current (I(A)) in different ways, exhibit leg shaking under ether anesthesia and have heightened metabolic rates and shortened life spans. We found that Sh, Hk, and eag mutant flies were all hypersensitive to paraquat. Double-mutant combinations among the three channel mutations and qvr(1) had drastically enhanced sensitivity to paraquat. Synaptic transmission at the larval neuromuscular junction was increased in the qvr(1) mutant to the level of Sh mutants. Similar to eag Sh double mutants, double mutants of eag and qvr(1) showed striking enhancement in synaptic transmission and a wings-down phenotype, the hallmarks of extreme hyperexcitability. Voltage-clamp experiments demonstrated that the qvr(1) mutation specifically disrupted the Sh-dependent I(A) current without altering the other currents [I(K), Ca(2+)-activated fast (I(CF)) and slow (I(CS)) currents, and I(Ca)] in larval muscles. Several deficiency strains of the qvr locus failed to complement qvr(1) and confirmed that ether-induced leg shaking, reduced I(A) current, and paraquat hypersensitivity map to the same locus. Our results suggest that the qvr gene may encode a novel K(+) channel-related polypeptide and indicate a strong link between a voltage-activated K(+) current and vulnerability to ROS.
Indexed as
Identifiers
10934243PMC6772572W1936722638What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.