ArticleThe Journal of experimental medicine2000
Cell-specific transcriptional regulation of human leukotriene B(4) receptor gene.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Analysis of DNA methylation at birth and in childhood reveals changes associated with season of birth and latitude.Clinical epigenetics · 2023Pooled it
- Role for BLT1 in regulating inflammation within adipose tissue immune cells of aged mice.Immunity & ageing : I & A · 2024Article
- Hepatic stellate cell stearoyl co-A desaturase activates leukotriene B4 receptor 2 - β-catenin cascade to promote liver tumorigenesis.Nature communications · 2023Article
- Review
- [Prognostic implications and functional enrichment analysis of LTB4R in patients with acute myeloid leukemia].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- LTB4 Promotes Acute Lung Injury via Upregulating the PLCDisease markers · 2022Article
- Expression of leukotriene BCellular & molecular immunology · 2021Article
- Leukotriene receptors as potential therapeutic targets.The Journal of clinical investigation · 2018Review
- Differential Contribution of BLT1 and BLT2 to Leukotriene B4-Induced Human NK Cell Cytotoxicity and Migration.Mediators of inflammation · 2015Article
- Evolutionary aspects of lipoxygenases and genetic diversity of human leukotriene signaling.Progress in lipid research · 2015Review
- Human-specific epigenetic variation in the immunological Leukotriene B4 Receptor (LTB4R/BLT1) implicated in common inflammatory diseases.Genome medicine · 2014Article
- Leukotriene B4 receptor locus gene characterisation and association studies in asthma.BMC medical genetics · 2012Article
- Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells.Journal of neuroinflammation · 2012Article
- Leukotriene B(4) BLT receptor signaling regulates the level and stability of cyclooxygenase-2 (COX-2) mRNA through restricted activation of Ras/Raf/ERK/p42 AUF1 pathway.The Journal of biological chemistry · 2010Article
- Leukotriene B4 release from mast cells in IgE-mediated airway hyperresponsiveness and inflammation.American journal of respiratory cell and molecular biology · 2009Article
- Differential induction of BLT receptor expression on human endothelial cells by lipopolysaccharide, cytokines, and leukotriene B4.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene.The Biochemical journal · 2006Article
- Leukotriene B4 signaling through NF-kappaB-dependent BLT1 receptors on vascular smooth muscle cells in atherosclerosis and intimal hyperplasia.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Requirement for leukotriene B4 receptor 1 in allergen-induced airway hyperresponsiveness.American journal of respiratory and critical care medicine · 2005Article
- 5-Lipoxygenase Pathway, Dendritic Cells, and Adaptive Immunity.Journal of biomedicine & biotechnology · 2004Article
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leukotriene B(4) (LTB(4)) is a lipid mediator that activates leukocytes and is involved in host defense and inflammation. BLT1, a high-affinity receptor for LTB(4) (originally termed BLT), is expressed exclusively in inflammatory cells and is inducible in macrophages upon activation. The mechanisms of tissue-specific expression and induction of BLT1 are important for the understanding of mechanism of onset and the potential treatment of inflammatory disorders. Here, we report the genomic structure and a promoter analysis of the human BLT1 gene, with an emphasis on the mechanism of cell-specific transcription. No TATA or CAAT elements exist around the transcription initiation sites, but a GC-rich sequence is observed in this region. A reporter gene assay revealed that a region approximately 80 basepair upstream from the initiator sequence is required for the basal transcription of the BLT1 gene. Sp1 was found to be a major activator of basal transcription by electrophoretic mobility shift assays and site-directed mutagenesis. The CpG sites of the BLT1 promoter region were highly methylated in BLT1-nonexpressing cells, but not methylated in BLT1-expressing cells. Further, methylation of this region in vitro inhibited the promoter activity to approximately 15% of the control. Thus, methylation at CpG sites in the promoter region is important for cell-specific transcription of the BLT1 gene. The promoter region of the BLT1 gene is localized within the open reading frame (ORF) of the BLT2 gene, which encodes a low-affinity receptor for LTB(4) (Yokomizo, T., K. Kato, K. Terawaki, T. Izumi, and T. Shimizu. 2000. J. Exp. Med. 192:421-431). To our knowledge, this is the first example of "promoter in ORF" in higher eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.