ArticleMolecular therapy : the journal of the American Society of Gene Therapy2000
Metabolic biotinylation of recombinant proteins in mammalian cells and in mice.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- Deciphering Spatial Protein-Protein Interactions in Brain Using Proximity Labeling.Molecular & cellular proteomics : MCP · 2022Review
- Mammalian Expression andACS omega · 2020Article
- Retargeting adenoviruses for therapeutic applications and vaccines.FEBS letters · 2020Review
- A novel biotinylated lipid raft reporter for electron microscopic imaging of plasma membrane microdomains.Journal of lipid research · 2012Article
- Selective cell-surface labeling of the molecular motor protein prestin.Biochemical and biophysical research communications · 2011Article
- An efficient vector system to modify cells genetically.PloS one · 2011Article
- Insights into GATA-1-mediated gene activation versus repression via genome-wide chromatin occupancy analysis.Molecular cell · 2009Article
- Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system.BMC biotechnology · 2008Article
- Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum.Biomolecular engineering · 2007Article
- Current advances and future challenges in Adenoviral vector biology and targeting.Current gene therapy · 2007Review
- Targeting EGFR with metabolically biotinylated fiber-mosaic adenovirus.Gene therapy · 2007Article
- Avidin-based targeting and purification of a protein IX-modified, metabolically biotinylated adenoviral vector.Molecular therapy : the journal of the American Society of Gene Therapy · 2004Article
- Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Metabolic biotinylation of recombinant proteins in mammalian cells and in mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2000Article
- Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform.Research in pharmaceutical sciencesArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The avidin-biotin system is a fundamental technology in biomedicine for immunolocalization, imaging, nucleic acid blotting, and protein labeling. While this technology is robust, it is limited by the fact that mammalian proteins must be expressed and purified prior to chemical biotinylation using cross-linking agents which modify proteins at random locations to heterogeneous levels and can inactivate protein function. To circumvent this limitation, we demonstrate the ability to metabolically biotinylate tagged proteins in mammalian cells and in mice using the endogenous biotinylation enzymes of the host. Endogenously biotinylated proteins were readily purified from mammalian cells using monomeric avidin and eluted under nondenaturing conditions using only biotin as the releasing agent. This technology should allow recombinant proteins and fragile protein complexes to be produced and purified from mammalian cells as well as from transgenic plants and animals. In addition, this technology may be particularly useful for cell-targeting applications in which proteins or viral gene therapy vectors can be biotinylated at genetically defined sites for combination with other targeting moieties complexed with avidin.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.