ReviewNeoplasia (New York, N.Y.)
Use of reporter genes for optical measurements of neoplastic disease in vivo.
Review in Neoplasia (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
108 citing papers in PubMed, 367 citations in OpenAlex.
- Establishment of KGAS, a cell line derived from gastric-type adenocarcinoma of the uterine cervix.Human cell · 2025Article
- Fluorescence Resonance Energy Transfer for Drug Loading Assessment in Reconstituted High-Density Lipoprotein Nanoparticles.International journal of molecular sciences · 2025Article
- Three-Dimensional Printer-Assisted Electrospinning for Fabricating Intricate Biological Tissue Mimics.Nanomaterials (Basel, Switzerland) · 2023Article
- Stored whole blood transfusion initiates serum amyloid A activation monitored by real-time dynamic imaging.Blood transfusion = Trasfusione del sangue · 2023Article
- Encapsulins: Structure, Properties, and Biotechnological Applications.Biochemistry. Biokhimiia · 2023Review
- Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.PloS one · 2023Article
- Imaging and detection of long-lived fluorescence probes in presence of highly emissive and scattering background.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- The Establishment of a Noninvasive Bioluminescence-Specific Viral Encephalitis Model by Pseudorabies Virus-Infected NF-κBp-Luciferase Mice.Veterinary sciences · 2022Article
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- In vivo long-term investigation of tumor bearing mKate2 by an in-house fluorescence molecular imaging system.Biomedical engineering online · 2018Article
- Dynamic imaging of the effect of mesenchymal stem cells on osteoclast precursor cell chemotaxis for bone defects in the mouse skull.Journal of dental sciences · 2018Article
- QualitativeBio-protocol · 2018Article
- Anti-prostate cancer activity of 8-hydroxyquinoline-2-carboxaldehyde-thiosemicarbazide copper complexes in vivo by bioluminescence imaging.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Thymoquinone Inhibits Bone Metastasis of Breast Cancer Cells Through Abrogation of the CXCR4 Signaling Axis.Frontiers in pharmacology · 2018Article
- Imaging of Tumor-Associated Macrophages in a Transgenic Mouse Model of Orthotopic Ovarian Cancer.Molecular imaging and biology · 2017Article
- Non-invasive imaging of engineered human tumors in the living chicken embryo.Scientific reports · 2017Article
- Stem Cell Monitoring with a Direct or Indirect Labeling Method.Nuclear medicine and molecular imaging · 2016Review
- Tumor Autonomous Effects of Vitamin D Deficiency Promote Breast Cancer Metastasis.Endocrinology · 2016Article
- Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage.Annals of biomedical engineering · 2016Review
- Alleviation of skin inflammation after Lin(-) cell transplantation correlates with their differentiation into myeloid-derived suppressor cells.Scientific reports · 2015Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Revealing the cellular and molecular changes associated with cancer, as they occur in intact living animal models of human neoplastic disease, holds tremendous potential for understanding disease mechanisms and elucidating effective therapies. Since light is transmitted through mammalian tissues, at a low level, optical signatures conferred on tumor cells by expression of reporter genes encoding bioluminescent and fluorescent proteins can be detected externally using sensitive photon detection systems. Expression of reporter genes, such as the bioluminescent enzyme firefly luciferase (Luc) or variants of green fluorescent protein (GFP) in transformed cells, can effectively be used to reveal molecular and cellular features of neoplasia in vivo. Tumor cell growth and regression in response to various therapies have been evaluated non-invasively in living experimental animals using these reporter genes. Detection of Luc-labeled cells in vivo was extremely sensitive with signals over background from as few as 1000 human tumor cells distributed throughout the peritoneal cavity of a mouse with linear relationships between cell number and signal intensity over five logs. GFP offers the strength of high-resolution ex vivo analyses following in vivo localization of the tumor. The dynamic range of Luc detection allows the full disease course to be monitored since disease progression from small numbers of cells to extensive disease can be assessed. As such, therapies that target minimal disease as well as those designed for late stage disease can be readily evaluated in animal models. Real time spatiotemporal analyses of tumor cell growth can reveal the dynamics of neoplastic disease, and facilitate rapid optimization of effective treatment regimens. Thus, these methods improve the predictability of animal models of human disease as study groups can be followed over time, and can accelerate the development of therapeutic strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.