ArticleNature biotechnology2000
Genetic selection for dissociative inhibitors of designated protein-protein interactions.
Article in Nature biotechnology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 63 citations in OpenAlex.
- De Novo Discovery of Bioactive Cyclic Peptides via Cellular Selection of Genetically Encoded Libraries.Chembiochem : a European journal of chemical biology · 2026Review
- CF2H: a cell-free two-hybrid platform for rapid protein binder screening.Nature communications · 2026Article
- Stepwise assembly of functional C-terminal REST/NRSF transcriptional repressor complexes as a drug target.Protein science : a publication of the Protein Society · 2017Article
- DNA-protein π-interactions in nature: abundance, structure, composition and strength of contacts between aromatic amino acids and DNA nucleobases or deoxyribose sugar.Nucleic acids research · 2014Article
- Identification of antituberculosis agents that target ribosomal protein interactions using a yeast two-hybrid system.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Mechanism of function of viral channel proteins and implications for drug development.International review of cell and molecular biology · 2012Review
- Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems.PloS one · 2012Article
- Bacteria-based in vivo peptide library screening using biopanning approach.Journal of microbiology (Seoul, Korea) · 2011Article
- Genetic selection for peptide inhibitors of angiogenin.Protein engineering, design & selection : PEDS · 2008Article
- Targeting human immunodeficiency virus type 1 assembly, maturation and budding.Drug target insights · 2007Article
- Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation.Nucleic acids research · 2005Article
- A systematic method for identifying small-molecule modulators of protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Screening peptide/protein libraries fused to the lambda repressor DNA-binding domain in E. coli cells.Methods in molecular biology (Clifton, N.J.) · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many biological processes rely on protein-protein interactions. These processes include signal transduction, cell cycle regulation, gene regulation, and viral assembly and replication. Moreover, many proteins and enzymes manifest their function as oligomers. We describe here an efficient means to sift through large combinatorial libraries and identify molecules that block the interaction of target proteins in vivo. The power of this approach is demonstrated by the identification of nine-residue peptides from a combinatorial library that inhibit the intracellular dimerization of HIV-1 protease. Fewer than 1 in 106 peptides do so. In vitro biochemical analyses of one such peptide demonstrate that it acts by dissociating HIV-1 protease into monomers, which are inactive catalysts. Inhibition is enhanced further by dimerizing the peptide. This approach enables the facile identification of new molecules that control cellular processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.