ArticleProceedings of the National Academy of Sciences of the United States of America2000
Action potentials reliably invade axonal arbors of rat neocortical neurons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 175 citations in OpenAlex.
- Biophysical properties of the membrane influence spike initiation dynamics and neuronal excitability: a focus on Kv1 channels in myelinated axons.Proceedings. Biological sciences · 2025Review
- Fast updating feedback from piriform cortex to the olfactory bulb relays multimodal identity and reward contingency signals during rule-reversal.Nature communications · 2025Article
- Axons compensate for biophysical constraints of variable size to uniformize their action potentials.PLoS biology · 2024Article
- Spike transmission failures in axons from cortical neuronsiScience · 2024Article
- Transthalamic Pathways for Cortical Function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Spike transmission failures in axons from mouse cortical pyramidal neurons in vivo.bioRxiv : the preprint server for biology · 2024Article
- Fast updating feedback from piriform cortex to the olfactory bulb relays multimodal reward contingency signals during rule-reversal.bioRxiv : the preprint server for biology · 2023Article
- Article
- Eavesdropping wires: Recording activity in axons using genetically encoded calcium indicators.Journal of neuroscience methods · 2021Review
- Neural anatomy and optical microscopy (NAOMi) simulation for evaluating calcium imaging methods.Journal of neuroscience methods · 2021Article
- Large, Stable Spikes Exhibit Differential Broadening in Excitatory and Inhibitory Neocortical Boutons.Cell reports · 2021Article
- InterpretingNeurophotonics · 2020Review
- Intermittent Failure of Spike Propagation in Primary Afferent Neurons during Tactile Stimulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- A Single MicroRNA-Hox Gene Module Controls Equivalent Movements in Biomechanically Distinct Forms of Drosophila.Current biology : CB · 2019Article
- Synaptotagmin-1 enables frequency coding by suppressing asynchronous release in a temperature dependent manner.Scientific reports · 2019Article
- Multichannel fiber photometry for mapping axonal terminal activity in a restricted brain region in freely moving mice.Neurophotonics · 2019Article
- In vivo measurement of afferent activity with axon-specific calcium imaging.Nature neuroscience · 2018Article
- In vivo wide-field calcium imaging of mouse thalamocortical synapses with an 8 K ultra-high-definition camera.Scientific reports · 2018Article
- The functional organization of cortical feedback inputs to primary visual cortex.Nature neuroscience · 2018Article
- Review
19 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Neocortical pyramidal neurons have extensive axonal arborizations that make thousands of synapses. Action potentials can invade these arbors and cause calcium influx that is required for neurotransmitter release and excitation of postsynaptic targets. Thus, the regulation of action potential invasion in axonal branches might shape the spread of excitation in cortical neural networks. To measure the reliability and extent of action potential invasion into axonal arbors, we have used two-photon excitation laser scanning microscopy to directly image action-potential-mediated calcium influx in single varicosities of layer 2/3 pyramidal neurons in acute brain slices. Our data show that single action potentials or bursts of action potentials reliably invade axonal arbors over a range of developmental ages (postnatal 10-24 days) and temperatures (24 degrees C-30 degrees C). Hyperpolarizing current steps preceding action potential initiation, protocols that had previously been observed to produce failures of action potential propagation in cultured preparations, were ineffective in modulating the spread of action potentials in acute slices. Our data show that action potentials reliably invade the axonal arbors of neocortical pyramidal neurons. Failures in synaptic transmission must therefore originate downstream of action potential invasion. We also explored the function of modulators that inhibit presynaptic calcium influx. Consistent with previous studies, we find that adenosine reduces action-potential-mediated calcium influx in presynaptic terminals. This reduction was observed in all terminals tested, suggesting that some modulatory systems are expressed homogeneously in most terminals of the same neuron.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.