ArticleProceedings of the National Academy of Sciences of the United States of America2000
Requirement for either a host- or pectin-induced pectate lyase for infection of Pisum sativum by Nectria hematococca.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 155 citations in OpenAlex.
- The role of pectate lyases in development and stress tolerance in plants.Molecular biology reports · 2025Review
- Community Structure, Growth-Promoting Potential, and Genomic Analysis of Seed-Endophytic Bacteria inMicroorganisms · 2025Article
- Transcriptomic analysis of Phytophthora infestans races and evaluation of their pathogenicity on potato.BMC plant biology · 2025Article
- Dispensable genome and segmental duplications drive the genome plasticity inFrontiers in fungal biology · 2025Article
- Horizontal transfers between fungal Fusarium species contributed to successive outbreaks of coffee wilt disease.PLoS biology · 2024Article
- Genome sequencing and comparative genomics reveal insights into pathogenicity and evolution of Fusarium zanthoxyli, the causal agent of stem canker in prickly ash.BMC genomics · 2024Article
- A plant cell death-inducing protein from litchi interacts with Peronophythora litchii pectate lyase and enhances plant resistance.Nature communications · 2024Article
- Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence.Microbiology spectrum · 2023Article
- Transcriptome sequencing leads to an improved understanding of the infection mechanism of Alternaria solani in potato.BMC plant biology · 2023Article
- Transcription Factor VdCf2 Regulates Growth, Pathogenicity, and the Expression of a Putative Secondary Metabolism Gene Cluster in Verticillium dahliae.Applied and environmental microbiology · 2022Article
- Comparative genomic and transcriptomic analyses of trans-kingdom pathogen Fusarium solani species complex reveal degrees of compartmentalization.BMC biology · 2022Article
- Comparative transcriptomic analysis of races 1, 2, 5 and 6 of Fusarium oxysporum f.sp. pisi in a susceptible pea host identifies differential pathogenicity profiles.BMC genomics · 2021Article
- Cytotoxic function of xylanase VdXyn4 in the plant vascular wilt pathogen Verticillium dahliae.Plant physiology · 2021Article
- The Role of Glycoside Hydrolases in Phytopathogenic Fungi and Oomycetes Virulence.International journal of molecular sciences · 2021Review
- Rye Snow Mold-AssociatedJournal of fungi (Basel, Switzerland) · 2020Article
- Genome-scale analyses and characteristics of putative pathogenicity genes of Stagonosporopsis cucurbitacearum, a pumpkin gummy stem blight fungus.Scientific reports · 2020Article
- Article
- FvSNF1, the sucrose non-fermenting protein kinase gene of Fusarium virguliforme, is required for cell-wall-degrading enzymes expression and sudden death syndrome development in soybean.Current genetics · 2017Article
- Polygalacturonase gene pgxB in Aspergillus niger is a virulence factor in apple fruit.PloS one · 2017Article
- Combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of Fusarium oxysporum.Molecular plant pathology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal pathogens usually have multiple genes that encode extracellular hydrolytic enzymes that may degrade the physical barriers in their hosts during the invasion process. Nectria hematococca, a plant pathogen, has two inducible pectate lyase (PL) genes (pel) encoding PL that can help degrade the carbohydrate barrier in the host. pelA is induced by pectin, whereas pelD is induced only in planta. We show that the disruption of either the pelA or pelD genes alone causes no detectable decrease in virulence. Disruption of both pelA and pelD drastically reduces virulence. Complementation of the double disruptant with pelD gene, or supplementation of the infection droplets of the double disruptant with either purified enzyme, PLA, or PLD, caused a recovery in virulence. These results show that PL is a virulence factor. Thus, we demonstrate that disruption of all functionally redundant genes is required to demonstrate the role of host barrier-degrading enzymes in pathogenesis and that dismissal of the role of such enzymes based on the effects of single-gene disruption may be premature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.