ArticleProceedings of the National Academy of Sciences of the United States of America2000
Interaction of pseudomonas aeruginosa with epithelial cells: identification of differentially regulated genes by expression microarray analysis of human cDNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 180 citations in OpenAlex.
- P. aeruginosa type III and type VI secretion systems modulate early response gene expression in type II pneumocytes in vitro.BMC genomics · 2022Article
- Gene expression in lung epithelial cells following interaction with Pneumocystis carinii and its specific life forms yields insights into host gene responses to infection.Microbiology and immunology · 2022Article
- NOX4 MediatesAntioxidants (Basel, Switzerland) · 2021Article
- Hippophae rhamnoides mediate gene expression profiles against keratinocytes infection of Staphylococcus aureus.Molecular biology reports · 2021Article
- CFTR Deficiency Affects Glucose Homeostasis via Regulating GLUT4 Plasma Membrane Transportation.Frontiers in cell and developmental biology · 2021Article
- Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells.Journal of visualized experiments : JoVE · 2020Article
- Cigarette smoke extract modulatesAmerican journal of physiology. Lung cellular and molecular physiology · 2020Article
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- Quorum quenching activity ofInfection and drug resistance · 2019Article
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- Choosing an appropriate infection model to study quorum sensing inhibition in Pseudomonas infections.International journal of molecular sciences · 2013Review
- Cyclic di-GMP signalling and the regulation of bacterial virulence.Microbiology (Reading, England) · 2013Review
- Transcriptome signature in young children with acute otitis media due to non-typeable Haemophilus influenzae.International immunology · 2013Article
- CFTR activity and mitochondrial function.Redox biology · 2013Review
- Transcriptome signature in young children with acute otitis media due to Streptococcus pneumoniae.Microbes and infection · 2012Article
- Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis.BMC genomics · 2012Article
- Early gene response of human brain microvascular endothelial cells to Listeria monocytogenes infection.Canadian journal of microbiology · 2011Article
- Contribution of the Rgg transcription regulator to metabolism and virulence of Streptococcus suis serotype 2.Infection and immunity · 2011Article
- Transcriptomic analysis of Escherichia coli O157:H7 and K-12 cultures exposed to inorganic and organic acids in stationary phase reveals acidulant- and strain-specific acid tolerance responses.Applied and environmental microbiology · 2010Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that plays a major role in lung function deterioration in cystic fibrosis patients. To identify critical host responses during infection, we have used high-density DNA microarrays, consisting of 1,506 human cDNA clones, to monitor gene expression in the A549 lung pneumocyte cell line during exposure to P. aeruginosa. We have identified host genes that are differentially expressed upon infection, several of which require interaction with P. aeruginosa and the expression of the major subunit of type IV pili, PilA. Differential expression of genes involved in various cellular functions was identified, and we selected the gene encoding the transcription factor interferon regulatory factor 1 (IRF-1) for further analysis. The levels of the IRF-1 transcript increased 3- to 4-fold in A549 cells after adherence by P. aeruginosa. A similar increase of IRF-1 mRNA was observed in A549 cells exposed to wild-type P. aeruginosa when compared to an isogenic, nonpiliated strain. However, this difference was abolished when serum was present during the incubation of bacteria. Exposure of A549 cells to purified P. aeruginosa lipopolysaccharide did not result in a significant increase in IRF-1 mRNA. Although the P. aeruginosa-induced increased IRF-1 expression depends on the presence of bacterial adhesin, our findings do not preclude the possibility that other bacterial products are responsible for IRF-1 activation, which is enhanced by bacterial adherence to cells. These data show that microarray technology can be an important tool for studying the complex interplay between bacterial pathogens and host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.