ArticleThe Journal of cell biology2000
Restructuring of focal adhesion plaques by PI 3-kinase. Regulation by PtdIns (3,4,5)-p(3) binding to alpha-actinin.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 130 citations in OpenAlex.
- Dietary Polyphenols Effects on Focal Adhesion Plaques and Metalloproteinases in Cancer Invasiveness.Biomedicines · 2024Review
- From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.Frontiers in cell and developmental biology · 2024Review
- Perturbed actin cap as a new personalized biomarker in primary fibroblasts of Huntington's disease patients.Frontiers in cell and developmental biology · 2023Article
- Kinetic Changes of Ptdins (3,4,5) P3 within Fast and Slow Turnover Rates of Focal Adhesion.Reports of biochemistry & molecular biology · 2022Article
- Thrombospondin-1 Signaling Through the Calreticulin/LDL Receptor Related Protein 1 Axis: Functions and Possible Roles in Glaucoma.Frontiers in cell and developmental biology · 2022Review
- Article
- Transcriptome profiling analysis of muscle tissue reveals potential candidate genes affecting water holding capacity in Chinese Simmental beef cattle.Scientific reports · 2021Article
- Rac1 Activity Is Modulated by Huntingtin and Dysregulated in Models of Huntington's Disease.Journal of Huntington's disease · 2019Article
- Huntingtin associates with the actin cytoskeleton and α-actinin isoforms to influence stimulus dependent morphology changes.PloS one · 2019Article
- The fifth sense: Mechanosensory regulation of alpha-actinin-4 and its relevance for cancer metastasis.Seminars in cell & developmental biology · 2017Review
- TCR Triggering Induces the Formation of Lck-RACK1-Actinin-1 Multiprotein Network Affecting Lck Redistribution.Frontiers in immunology · 2016Article
- Modulation of the extracellular matrix patterning of thrombospondins by actin dynamics and thrombospondin oligomer state.Bioscience reports · 2015Article
- Integrin-dependent force transmission to the extracellular matrix by α-actinin triggers adhesion maturation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Alpha-actinin 4 and tumorigenesis of breast cancer.Vitamins and hormones · 2013Review
- Thrombospondin1 in tissue repair and fibrosis: TGF-β-dependent and independent mechanisms.Matrix biology : journal of the International Society for Matrix Biology · 2012Review
- Regulation of local expression of cell adhesion and motility-related mRNAs in breast cancer cells by IMP1/ZBP1.Journal of cell science · 2012Article
- The thrombospondins.Cold Spring Harbor perspectives in biology · 2011Review
- Kindlin-2 regulates podocyte adhesion and fibronectin matrix deposition through interactions with phosphoinositides and integrins.Journal of cell science · 2011Article
- What is vinculin needed for in platelets?Journal of thrombosis and haemostasis : JTH · 2010Article
- Myosin II directly binds and inhibits Dbl family guanine nucleotide exchange factors: a possible link to Rho family GTPases.The Journal of cell biology · 2010Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Focal adhesions are an elaborate network of interconnecting proteins linking actin stress fibers to the extracellular matrix substrate. Modulation of the focal adhesion plaque provides a mechanism for the regulation of cellular adhesive strength. Using interference reflection microscopy, we found that activation of phosphoinositide 3-kinase (PI 3-kinase) by PDGF induces the dissipation of focal adhesions. Loss of this close apposition between the cell membrane and the extracellular matrix coincided with a redistribution of alpha-actinin and vinculin from the focal adhesion complex to the Triton X-100-soluble fraction. In contrast, talin and paxillin remained localized to focal adhesions, suggesting that activation of PI 3-kinase induced a restructuring of the plaque rather than complete dispersion. Furthermore, phosphatidylinositol (3,4, 5)-trisphosphate (PtdIns (3,4,5)-P(3)), a lipid product of PI 3-kinase, was sufficient to induce restructuring of the focal adhesion plaque. We also found that PtdIns (3,4,5)-P(3) binds to alpha-actinin in PDGF-treated cells. Further evidence demonstrated that activation of PI 3-kinase by PDGF induced a decrease in the association of alpha-actinin with the integrin beta subunit, and that PtdIns (3,4,5)-P(3) could disrupt this interaction in vitro. Modification of focal adhesion structure by PI 3-kinase and its lipid product, PtdIns (3,4,5)-P(3), has important implications for the regulation of cellular adhesive strength and motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.