ArticleMolecular biology of the cell2000
Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 208 citations in OpenAlex.
- Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans.The Journal of cell biology · 2026Article
- Fission yeast Pdk1 kinase regulates cytokinesis and eisosomes.Molecular biology of the cell · 2025Article
- Fission yeast Pdk1 kinase regulates cytokinesis and eisosomes.bioRxiv : the preprint server for biology · 2025Article
- PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast.Molecular biology of the cell · 2025Article
- Characterization of temperature-sensitive alleles of anillin-like Mid1 and polo kinase Plo1 inmicroPublication biology · 2025Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Processes Controlling the Contractile Ring during Cytokinesis in Fission Yeast, Including the Role of ESCRT Proteins.Journal of fungi (Basel, Switzerland) · 2024Review
- Anillin-related Mid1 as an adaptive and multimodal contractile ring anchoring protein: A simulation study.Structure (London, England : 1993) · 2024Article
- Design principles of Cdr2 node patterns in fission yeast cells.Molecular biology of the cell · 2023Article
- Design principles of Cdr2 node patterns in fission yeast cells.bioRxiv : the preprint server for biology · 2023Article
- Article
- Anillin/Mid1p interacts with the ESCRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast.Cell cycle (Georgetown, Tex.) · 2021Article
- Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.The Journal of cell biology · 2021Article
- Connecting cell polarity signals to the cytokinetic machinery in yeast and metazoan cells.Cell cycle (Georgetown, Tex.) · 2021Review
- Fission yeast Pak1 phosphorylates anillin-like Mid1 for spatial control of cytokinesis.The Journal of cell biology · 2020Article
- Comparative Analysis of the Roles of Non-muscle Myosin-IIs in Cytokinesis in Budding Yeast, Fission Yeast, and Mammalian Cells.Frontiers in cell and developmental biology · 2020Review
- Article
- Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe.Molecular microbiology · 2019Review
- The Functionally Important N-Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation.Biochemistry · 2019Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
42 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
mid1 is required for the proper placement of the contractile actin ring for cytokinesis at a medial site overlying the nucleus. Here we find that mid1 protein (mid1p) shuttles between the nucleus and a cortical medial broad band during interphase and early mitosis. The position of this broad band, which overlies the nucleus, is linked to nuclear position even in cells with displaced or multiple nuclei. We identified and created mutations in an NLS and in two crm1-dependent NES sequences in mid1p. NES mutations caused mid1p accumulation in the nucleus and loss of function. An NLS mutations greatly reduced nuclear localization but did not perturb cytoplasmic localization or function. mid1p localization to the medial broad band was also not dependent on mid1p PH domain or microtubule and actin cytoskeletons. Overexpression of mid1p produced ectopic cell growth at this band during interphase and abnormal karmellae-like nuclear membrane structures. In plo1-1, mid1p formed a medial broad band but did not incorporate into a tight ring, suggesting that polo kinase plo1p is required for activation of mid1p function. Thus, the mid1p broad band defines a compartment at the medial cell surface, whose localization is linked to the position of the nucleus, and whose function may be to position the plane of cell division.
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Registered trials
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