ArticleMolecular biology of the cell2000
Nuclear dynamics in Arabidopsis thaliana.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed.
- A Method to Visualize Cell Proliferation ofPlant direct · 2025Article
- Mechanical resistance of the environment affects root hair growth and nucleus dynamics.Scientific reports · 2024Article
- The nuclear pore Y-complex functions as a platform for transcriptional regulation of FLOWERING LOCUS C in Arabidopsis.The Plant cell · 2024Article
- The nuclear lamina is required for proper development and nuclear shape distortion in tomato.Journal of experimental botany · 2023Article
- Modeling the 3D genome of plants.Nucleus (Austin, Tex.) · 2021Article
- Vacuolar occupancy is crucial for cell elongation and growth regardless of the underlying mechanism.Plant signaling & behavior · 2021Article
- Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression.Plant cell reports · 2021Article
- Regulation of diverse nuclear shapes: pathways working independently, together.Communicative & integrative biology · 2021Review
- Regulation and Physiological Significance of the Nuclear Shape in Plants.Frontiers in plant science · 2021Review
- Automated 3D bio-imaging analysis of nuclear organization by NucleusJ 2.0.Nucleus (Austin, Tex.) · 2020Article
- Advancing knowledge of the plant nuclear periphery and its application for crop science.Nucleus (Austin, Tex.) · 2020Review
- Cytosolic calcium localization and dynamics during early endosperm development in the genus Agave (Asparagales, Asparagaceae).Protoplasma · 2019Article
- Loss of CRWN Nuclear Proteins Induces Cell Death and Salicylic Acid Defense Signaling.Plant physiology · 2019Article
- Polar vacuolar distribution is essential for accurate asymmetric division ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Auxin and ROP GTPase Signaling of Polar Nuclear Migration in Root Epidermal Hair Cells.Plant physiology · 2018Article
- The WASP-Arp2/3 complex signal cascade is involved in actin-dependent sperm nuclei migration during double fertilization in tobacco and maize.Scientific reports · 2017Article
- PCaP2 regulates nuclear positioning in growing Arabidopsis thaliana root hairs by modulating filamentous actin organization.Plant cell reports · 2015Article
- Moving and positioning the nucleus in skeletal muscle - one step at a time.Nucleus (Austin, Tex.) · 2015Review
- Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy.Frontiers in plant science · 2015Article
- Plant nuclear shape is independently determined by the SUN-WIP-WIT2-myosin XI-i complex and CRWN1.Nucleus (Austin, Tex.) · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleus is a definitive feature of eukaryotic cells, comprising twin bilamellar membranes, the inner and outer nuclear membranes, which separate the nucleoplasmic and cytoplasmic compartments. Nuclear pores, complex macromolecular assemblies that connect the two membranes, mediate communication between these compartments. To explore the morphology, topology, and dynamics of nuclei within living plant cells, we have developed a novel method of confocal laser scanning fluorescence microscopy under time-lapse conditions. This is used for the examination of the transgenic expression in Arabidopsis thaliana of a chimeric protein, comprising the GFP (Green-Fluorescent Protein of Aequorea victoria) translationally fused to an effective nuclear localization signal (NLS) and to beta-glucuronidase (GUS) from E. coli. This large protein is targeted to the nucleus and accumulates exclusively within the nucleoplasm. This article provides online access to movies that illustrate the remarkable and unusual properties displayed by the nuclei, including polymorphic shape changes and rapid, long-distance, intracellular movement. Movement is mediated by actin but not by tubulin; it therefore appears distinct from mechanisms of nuclear positioning and migration that have been reported for eukaryotes. The GFP-based assay is simple and of general applicability. It will be interesting to establish whether the novel type of dynamic behavior reported here, for higher plants, is observed in other eukaryotic organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.