ArticleMolecular biology of the cell2000
The interaction between Ran and NTF2 is required for cell cycle progression.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Genome-wide screening of mitogen-activated protein kinase (MAPK) gene family and expression profile under heavy metal stress in Solanum lycopersicum.Biotechnology letters · 2025Article
- A phylogenetic study of the members of the MAPK and MEK families across Viridiplantae.PloS one · 2021Article
- Genome-wide mapping of Myc binding and gene regulation in serum-stimulated fibroblasts.Oncogene · 2012Article
- Identification and characterization of the Arabidopsis FG-repeat nucleoporin Nup62.Plant signaling & behavior · 2011Article
- Function of nuclear transport factor 2 and Ran in the 20E signal transduction pathway in the cotton bollworm, Helicoverpa armigera.BMC cell biology · 2010Article
- The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling.The Plant cell · 2007Article
- Article
- Identification and characterization of the Arabidopsis orthologs of nuclear transport factor 2, the nuclear import factor of ran.Plant physiology · 2006Article
- Article
- Ran GTPase regulates Mad2 localization to the nuclear pore complex.Eukaryotic cell · 2005Article
- Part of Ran is associated with AKAP450 at the centrosome: involvement in microtubule-organizing activity.Molecular biology of the cell · 2003Article
- Yaf9, a novel NuA4 histone acetyltransferase subunit, is required for the cellular response to spindle stress in yeast.Molecular and cellular biology · 2003Article
- Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.Molecular biology of the cell · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The small GTPase Ran is required for the trafficking of macromolecules into and out of the nucleus. Ran also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, Ran is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. Nuclear transport factor 2 (NTF2) has been shown to import RanGDP into the nucleus. Here, we examine the in vivo role of NTF2 in Ran import and the effect that disruption of Ran imported into the nucleus has on the cell cycle. A temperature-sensitive (ts) mutant of Saccharomyces cerevisiae NTF2 that does not bind to Ran is unable to import Ran into the nucleus at the nonpermissive temperature. Moreover, when Ran is inefficiently imported into the nucleus, cells arrest in G(2) in a MAD2 checkpoint-dependent manner. These findings demonstrate that NTF2 is required to transport Ran into the nucleus in vivo. Furthermore, we present data that suggest that depletion of nuclear Ran triggers a spindle-assembly checkpoint-dependent cell cycle arrest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.