ArticleMolecular biology of the cell2000
The AP-3 complex required for endosomal synaptic vesicle biogenesis is associated with a casein kinase Ialpha-like isoform.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
- Formation, Organization, and Maintenance of Synaptic Vesicle Pools: From Molecules to Structure and Dynamics.Advances in neurobiology · 2026Review
- LRK-1/LRRK2 and AP-3 regulate trafficking of synaptic vesicle precursors through active zone protein SYD-2/Liprin-α.PLoS genetics · 2024Article
- Inositol hexakisphosphate kinases differentially regulate trafficking of vesicular glutamate transporters 1 and 2.Frontiers in cellular neuroscience · 2022Article
- AP-3 adaptor complex-mediated vesicle trafficking.Biophysics reports · 2021Article
- UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes.PLoS genetics · 2017Article
- LRRK2 and RAB7L1 coordinately regulate axonal morphology and lysosome integrity in diverse cellular contexts.Scientific reports · 2016Article
- Variation in PTCHD2, CRISP3, NAP1L4, FSCB, and AP3B2 associated with spherical equivalent.Molecular vision · 2016Article
- Contributions of Bcl-xL to acute and long term changes in bioenergetics during neuronal plasticity.Biochimica et biophysica acta · 2014Review
- Adaptor protein complexes and intracellular transport.Bioscience reports · 2014Review
- The CK1 Family: Contribution to Cellular Stress Response and Its Role in Carcinogenesis.Frontiers in oncology · 2014Review
- Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.Molecular biology of the cell · 2013Article
- Article
- Suppression of α-synuclein toxicity and vesicle trafficking defects by phosphorylation at S129 in yeast depends on genetic context.Human molecular genetics · 2012Article
- Vesicular zinc regulates the Ca2+ sensitivity of a subpopulation of presynaptic vesicles at hippocampal mossy fiber terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.Molecular biology of the cell · 2011Article
- CK1 activates minus-end-directed transport of membrane organelles along microtubules.Molecular biology of the cell · 2011Article
- Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Inositol pyrophosphate mediated pyrophosphorylation of AP3B1 regulates HIV-1 Gag release.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.PLoS biology · 2009Article
- Genome-wide analysis of AP-3-dependent protein transport in yeast.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The formation of small vesicles is mediated by cytoplasmic coats the assembly of which is regulated by the activity of GTPases, kinases, and phosphatases. A heterotetrameric AP-3 adaptor complex has been implicated in the formation of synaptic vesicles from PC12 endosomes (). When the small GTPase ARF1 is prevented from hydrolyzing GTP, we can reconstitute AP-3 recruitment to synaptic vesicle membranes in an assembly reaction that requires temperatures above 15 degrees C and the presence of ATP suggesting that an enzymatic step is involved in the coat assembly. We have now found an enzymatic reaction, the phosphorylation of the AP-3 adaptor complex, that is linked with synaptic vesicle coating. Phosphorylation occurs in the beta3 subunit of the complex by a kinase similar to casein kinase 1alpha. The kinase copurifies with neuronal-specific AP-3. In vitro, purified casein kinase I selectively phosphorylates the beta3A and beta3B subunit at its hinge domain. Inhibiting the kinase hinders the recruitment of AP-3 to synaptic vesicles. The same inhibitors that prevent coat assembly in vitro also inhibit the formation of synaptic vesicles in PC12 cells. The data suggest, therefore, that the mechanism of AP-3-mediated vesiculation from neuroendocrine endosomes requires the phosphorylation of the adaptor complex at a step during or after AP-3 recruitment to membranes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.