ArticleMolecular biology of the cell2000
Role of p97 and syntaxin 5 in the assembly of transitional endoplasmic reticulum.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 101 citations in OpenAlex.
- Loss of function of VCP/TER94 causes neurodegeneration.Disease models & mechanisms · 2024Article
- ATPase Valosin-Containing Protein (VCP) Is Involved During the Replication and Egress of Sialodacryoadenitis Virus (SDAV) in Neurons.International journal of molecular sciences · 2024Article
- Orthodenticle homeobox 2 is transported to lysosomes by nuclear budding vesicles.Nature communications · 2023Article
- [Size exclusion-reverse liquid column chromatography-mass spectrometry and its application in the identification of post-translationally modified proteins in rat kidney].Se pu = Chinese journal of chromatography · 2021Article
- An intrinsically disordered motif regulates the interaction between the p47 adaptor and the p97 AAA+ ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The involvement of endoplasmic reticulum formation and protein synthesis efficiency in VCP- and ATL1-related neurological disorders.Journal of biomedical science · 2018Review
- Roles for the VCP co-factors Npl4 and Ufd1 in neuronal function in Drosophila melanogaster.Journal of genetics and genomics = Yi chuan xue bao · 2017Article
- A Mighty "Protein Extractor" of the Cell: Structure and Function of the p97/CDC48 ATPase.Frontiers in molecular biosciences · 2017Review
- UBXD Proteins: A Family of Proteins with Diverse Functions in Cancer.International journal of molecular sciences · 2016Review
- VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation.Nature communications · 2016Article
- Fission and fusion of the neuronal endoplasmic reticulum.Translational stroke research · 2013Review
- Valosin-containing protein (VCP/p97) is required for poliovirus replication and is involved in cellular protein secretion pathway in poliovirus infection.Journal of virology · 2012Article
- Loss of soluble N-ethylmaleimide-sensitive factor attachment protein α (αSNAP) induces epithelial cell apoptosis via down-regulation of Bcl-2 expression and disruption of the Golgi.The Journal of biological chemistry · 2012Article
- p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO.Nature communications · 2012Article
- Positive cooperativity of the p97 AAA ATPase is critical for essential functions.The Journal of biological chemistry · 2011Article
- AAA ATPase p97/VCP: cellular functions, disease and therapeutic potential.Journal of cellular and molecular medicine · 2008Article
- In planta analysis of the cell cycle-dependent localization of AtCDC48A and its critical roles in cell division, expansion, and differentiation.Plant physiology · 2008Article
- Topology of molecular machines of the endoplasmic reticulum: a compilation of proteomics and cytological data.Histochemistry and cell biology · 2008Review
- RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics.PLoS pathogens · 2006Article
- Nm23H2 facilitates coat protein complex II assembly and endoplasmic reticulum export in mammalian cells.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transitional endoplasmic reticulum (tER) consists of confluent rough and smooth endoplasmic reticulum (ER) domains. In a cell-free incubation system, low-density microsomes (1.17 g cc(-1)) isolated from rat liver homogenates reconstitute tER by Mg(2+)GTP- and Mg(2+)ATP-hydrolysis-dependent membrane fusion. The ATPases associated with different cellular activities protein p97 has been identified as the relevant ATPase. The ATP depletion by hexokinase or treatment with either N-ethylmaleimide or anti-p97 prevented assembly of the smooth ER domain of tER. High-salt washing of low-density microsomes inhibited assembly of the smooth ER domain of tER, whereas the readdition of purified p97 with associated p47 promoted reconstitution. The t-SNARE syntaxin 5 was observed within the smooth ER domain of tER, and antisyntaxin 5 abrogated formation of this same membrane compartment. Thus, p97 and syntaxin 5 regulate assembly of the smooth ER domain of tER and hence one of the earliest membrane differentiated components of the secretory pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.