ArticleMolecular biology of the cell2000
Critical activities of Rac1 and Cdc42Hs in skeletal myogenesis: antagonistic effects of JNK and p38 pathways.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 112 citations in OpenAlex.
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- CDC50A is required for aminophospholipid transport and cell fusion in mouse C2C12 myoblasts.Journal of cell science · 2022Article
- Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.Frontiers in physiology · 2021Article
- Stochastic Dynamics of Gene Switching and Energy Dissipation for Gene Expression.Frontiers in genetics · 2020Article
- DEPDC1B is a key regulator of myoblast proliferation in mouse and man.Cell proliferation · 2020Article
- Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation.Scientific reports · 2019Article
- Expression profiling of lncRNAs and mRNAs reveals regulation of muscle growth in the Pacific abalone, Haliotis discus hannai.Scientific reports · 2018Article
- FoxO1: a novel insight into its molecular mechanisms in the regulation of skeletal muscle differentiation and fiber type specification.Oncotarget · 2017Review
- Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion.International journal of molecular sciences · 2016Article
- FOXO1 Mediates Vitamin D Deficiency-Induced Insulin Resistance in Skeletal Muscle.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- Phospholipase D1 facilitates second-phase myoblast fusion and skeletal muscle regeneration.Molecular biology of the cell · 2015Article
- CEP2 attenuates myoblast differentiation but does not affect proliferation.International journal of biological sciences · 2015Article
- p104 binds to Rac1 and reduces its activity during myotube differentiation of C2C12 cell.TheScientificWorldJournal · 2014Article
- Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.Skeletal muscle · 2013Article
- Localization and function of Xinα in mouse skeletal muscle.American journal of physiology. Cell physiology · 2013Article
- Signaling mechanisms in mammalian myoblast fusion.Science signaling · 2013Review
- P-cadherin is a direct PAX3-FOXO1A target involved in alveolar rhabdomyosarcoma aggressiveness.Oncogene · 2013Article
- Nuclear movement during myotube formation is microtubule and dynein dependent and is regulated by Cdc42, Par6 and Par3.EMBO reports · 2012Article
- MicroRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rho family of GTP-binding proteins plays a critical role in a variety of cellular processes, including cytoskeletal reorganization and activation of kinases such as p38 and C-jun N-terminal kinase (JNK) MAPKs. We report here that dominant negative forms of Rac1 and Cdc42Hs inhibit the expression of the muscle-specific genes myogenin, troponin T, and myosin heavy chain in L6 and C2 myoblasts. Such inhibition correlates with decreased p38 activity. Active RhoA, RhoG, Rac1, and Cdc42Hs also prevent myoblast-to-myotube transition but affect distinct stages: RhoG, Rac1, and Cdc42Hs inhibit the expression of all muscle-specific genes analyzed, whereas active RhoA potentiates their expression but prevents the myoblast fusion process. We further show by two different approaches that the inhibitory effects of active Rac1 and Cdc42Hs are independent of their morphogenic activities. Rather, myogenesis inhibition is mediated by the JNK pathway, which also leads to a cytoplasmic redistribution of Myf5. We propose that although Rho proteins are required for the commitment of myogenesis, they differentially influence this process, positively for RhoA and Rac1/Cdc42Hs through the activation of the SRF and p38 pathways, respectively, and negatively for Rac1/Cdc42Hs through the activation of the JNK pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.