ArticleThe Journal of clinical investigation2000
In vivo alterations of IFN regulatory factor-1 and PIAS1 protein levels in cystic fibrosis epithelium.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Comparative transcriptome analysis unveils the adaptative mechanisms ofComputational and structural biotechnology journal · 2020Article
- Newborn Cystic Fibrosis Pigs Have a Blunted Early Response to an Inflammatory Stimulus.American journal of respiratory and critical care medicine · 2016Article
- Ibuprofen regulation of microtubule dynamics in cystic fibrosis epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Regulatory role of β-arrestin-2 in cholesterol processing in cystic fibrosis epithelial cells.Journal of lipid research · 2012Article
- Cystic fibrosis: a mucosal immunodeficiency syndrome.Nature medicine · 2012Review
- Genetic variation and clinical heterogeneity in cystic fibrosis.Annual review of pathology · 2012Review
- β-arrestin-2 regulation of the cAMP response element binding protein.Biochemistry · 2011Article
- Genomewide association analysis of respiratory syncytial virus infection in mice.Journal of virology · 2010Article
- IFN{gamma} regulates retinal pigment epithelial fluid transport.American journal of physiology. Cell physiology · 2009Article
- High-content functional screen to identify proteins that correct F508del-CFTR function.Molecular & cellular proteomics : MCP · 2009Article
- Anti-inflammatory therapies for cystic fibrosis-related lung disease.Clinical reviews in allergy & immunology · 2008Review
- cAMP-mediated regulation of cholesterol accumulation in cystic fibrosis and Niemann-Pick type C cells.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- DNA demethylation-dependent enhancement of toll-like receptor-2 gene expression in cystic fibrosis epithelial cells involves SP1-activated transcription.BMC molecular biology · 2008Article
- Chronic inflammation in the cystic fibrosis lung: alterations in inter- and intracellular signaling.Clinical reviews in allergy & immunology · 2008Review
- Sputum biomarkers of inflammation in cystic fibrosis lung disease.Proceedings of the American Thoracic Society · 2007Review
- Anaplasma phagocytophilum modulates gp91phox gene expression through altered interferon regulatory factor 1 and PU.1 levels and binding of CCAAT displacement protein.Infection and immunity · 2005Article
- State of the art: why do the lungs of patients with cystic fibrosis become infected and why can't they clear the infection?Respiratory research · 2003Review
- Regulation of airway tight junctions by proinflammatory cytokines.Molecular biology of the cell · 2002Article
- Monitoring inflammation in CF. Cytokines.Clinical reviews in allergy & immunology · 2002Review
- Pulmonary inflammation induced by Pseudomonas aeruginosa lipopolysaccharide, phospholipase C, and exotoxin A: role of interferon regulatory factor 1.Infection and immunity · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inducible nitric oxide synthase-2 (NOS2) expression has been shown to be reduced in cystic fibrosis (CF) epithelial cells. Reduced NOS2 expression is unexpected, given the inflammatory nature of CF airway disease, and is an indication that cell-signaling mechanisms necessary for proper NOS2 regulation are probably altered in CF epithelium. Therefore, we examined the expression levels of regulatory factors necessary for NOS2 expression in CF epithelium and showed that IFN regulatory factor-1 (IRF-1) is necessary for full NOS2 expression. Mice lacking IRF-1 expression have diminished epithelial NOS2 expression, as well as reduced NO-dependent chloride transport across the nasal epithelia. Furthermore, IRF-1 protein expression is reduced in nasal and intestinal epithelial cells from CF mice, suggesting a possible mechanism for the CF-related reduction of epithelial NOS2 expression. Active signal transducer and activator of transcription-1 (Stat1) is necessary for both NOS2 and IRF-1 expression. We found that protein levels of Stat1 were increased in CF cells, but that the active phosphorylated form of Stat1 was bound to the protein inhibitor of activated Stat1 (PIAS1). We propose that increased levels of PIAS1 diminish certain cell-signaling pathways, resulting in reduced IRF-1 and NOS2 expression in CF epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.