ArticleGenetics2000
The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis.The New phytologist · 2026Article
- Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.The Plant cell · 2025Review
- Agrobacterium-Mediated Genetic Transformation in Wheat.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat.Plants (Basel, Switzerland) · 2022Review
- Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.BMC plant biology · 2019Article
- A Dual sgRNA Approach for Functional Genomics inG3 (Bethesda, Md.) · 2018Article
- Opportunities for Innovation in Genetic Transformation of Forest Trees.Frontiers in plant science · 2018Review
- An in planta biolistic method for stable wheat transformation.Scientific reports · 2017Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.Genome biology · 2015Article
- Article
- Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.Plant cell reports · 2013Article
- The classical arabinogalactan protein AGP18 mediates megaspore selection in Arabidopsis.The Plant cell · 2013Article
- Functional analysis of sporophytic transcripts repressed by the female gametophyte in the ovule of Arabidopsis thaliana.PloS one · 2013Article
- Polycomb group proteins are required to couple seed coat initiation to fertilization.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette.Journal of Zhejiang University. Science. B · 2009Article
- Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana.Plant cell reports · 2009Article
- Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens.Plant cell reports · 2009Article
- An intensive understanding of vacuum infiltration transformation of pakchoi (Brassica rapa ssp. chinensis).Plant cell reports · 2008Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In planta transformation methods are now commonly used to transform Arabidopsis thaliana by Agrobacterium tumefaciens. The origin of transformants obtained by these methods has been studied by inoculating different floral stages and examining gametophytic expression of an introduced beta-glucuronidase marker gene encoding GUS. We observed that transformation can still occur after treating flowers where embryo sacs have reached the stage of the third division. No GUS expression was observed in embryo sacs or pollen of plants infiltrated with an Agrobacterium strain bearing a GUS gene under the control of a gametophyte-specific promoter. To identify the genetic target we used an insertion mutant in which a gene essential for male gametophytic development has been disrupted by a T-DNA bearing a Basta resistance gene (B(R)). In this mutant the B(R) marker is transferred to the progeny only by the female gametes. This mutant was retransformed with a hygromycin resistance marker and doubly resistant plants were selected. The study of 193 progeny of these transformants revealed 25 plants in which the two resistance markers were linked in coupling and only one plant where they were linked in repulsion. These results point to the chromosome set of the female gametophyte as the main target for the T-DNA.
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