ArticleGenetics2000
Genetic analysis of the role of Pol II holoenzyme components in repression by the Cyc8-Tup1 corepressor in yeast.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Repression by theeLife · 2021Article
- Epigenetic Transcriptional Memory ofGenetics · 2017Article
- Mediator Undergoes a Compositional Change during Transcriptional Activation.Molecular cell · 2016Article
- The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene.Biochimica et biophysica acta · 2014Article
- Analysis of Candida albicans mutants defective in the Cdk8 module of mediator reveal links between metabolism and biofilm formation.PLoS genetics · 2014Article
- Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.The EMBO journal · 2012Article
- The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.Genes & development · 2011Article
- Shields up: the Tup1-Cyc8 repressor complex blocks coactivator recruitment.Genes & development · 2011Article
- Review
- The LAMMER kinase homolog, Lkh1, regulates Tup transcriptional repressors through phosphorylation in Schizosaccharomyces pombe.The Journal of biological chemistry · 2010Article
- Spreading of a corepressor linked to action of long-range repressor hairy.Molecular and cellular biology · 2008Article
- Reciprocal nuclear shuttling of two antagonizing Zn finger proteins modulates Tup family corepressor function to repress chromatin remodeling.Eukaryotic cell · 2006Article
- Synergy among differentially regulated repressors of the ribonucleotide diphosphate reductase genes of Saccharomyces cerevisiae.Eukaryotic cell · 2006Article
- Glucose signaling in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo.Molecular and cellular biology · 2005Article
- Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.Molecular biology of the cell · 2005Article
- Functional comparison of the Tup11 and Tup12 transcriptional corepressors in fission yeast.Molecular and cellular biology · 2005Article
- Glucose repression of STA1 expression is mediated by the Nrg1 and Sfl1 repressors and the Srb8-11 complex.Molecular and cellular biology · 2004Article
- Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae.Molecular biology of the cell · 2004Article
- Fission yeast global repressors regulate the specificity of chromatin alteration in response to distinct environmental stresses.Nucleic acids research · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Cyc8-Tup1 corepressor complex is targeted to promoters by pathway-specific DNA-binding repressors, thereby inhibiting the transcription of specific classes of genes. Genetic screens have identified mutations in a variety of Pol II holoenzyme components (Srb8, Srb9, Srb10, Srb11, Sin4, Rgr1, Rox3, and Hrs1) and in the N-terminal tails of histones H3 and H4 that weaken repression by Cyc8-Tup1. Here, we analyze the effect of individual and multiple mutations in many of these components on transcriptional repression of natural promoters that are regulated by Cyc8-Tup1. In all cases tested, individual mutations have a very modest effect on SUC2 RNA levels and no detectable effect on levels of ANB1, MFA2, and RNR2. Furthermore, multiple mutations within the Srb components, between Srbs and Sin4, and between Srbs and histone tails affect Cyc8-Tup1 repression to the same modest extent as the individual mutations. These results argue that the weak effects of the various mutations on repression by Cyc8-Tup1 are not due to redundancy among components of the Pol II machinery, and they argue against a simple redundancy between the holoenzyme and chromatin pathways. In addition, phenotypic analysis indicates that, although Srbs8-11 are indistinguishable with respect to Cyc8-Tup1 repression, the individual Srbs are functionally distinct in other respects. Genetic interactions among srb mutations imply that a balance between the activities of Srb8 + Srb10 and Srb11 is important for normal cell growth.
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