ArticleProceedings of the National Academy of Sciences of the United States of America2000
3' poly(A) is dispensable for translation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- The yeast mitochondrial porin represses Snf1/AMP kinase signaling to attenuate viral replication.Genetics · 2026Article
- Pan2-Pan3 Complex-Mediated Deadenylation Enforces mRNA Quality Control for Infection of the Rice Blast Fungus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Annotation and functional prediction of RNA helicases inIMA fungus · 2025Review
- Diverse yeast antiviral systems prevent lethal pathogenesis caused by the L-A mycovirus.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Ribosome states signal RNA quality control.Molecular cell · 2021Review
- Apolipoprotein C-III and cardiovascular diseases: when genetics meet molecular pathologies.Molecular biology reports · 2021Review
- NONU-1 Encodes a Conserved Endonuclease Required for mRNA Translation Surveillance.Cell reports · 2020Article
- Ubiquitination of stalled ribosome triggers ribosome-associated quality control.Nature communications · 2017Article
- Tick-borne pathogens induce differential expression of genes promoting cell survival and host resistance in Ixodes ricinus cells.Parasites & vectors · 2017Article
- Rotavirus NSP3 Is a Translational Surrogate of the Poly(A) Binding Protein-Poly(A) Complex.Journal of virology · 2015Article
- Viruses and prions of Saccharomyces cerevisiae.Advances in virus research · 2013Review
- Discovering protein-based inheritance through yeast genetics.The Journal of biological chemistry · 2012Review
- The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1.Nucleic acids research · 2011Article
- Yeast double-stranded RNA virus L-A deliberately synthesizes RNA transcripts with 5'-diphosphate.The Journal of biological chemistry · 2010Article
- Article
- Launching the yeast 23S RNA Narnavirus shows 5' and 3' cis-acting signals for replication.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A cell-free protein synthesis system for high-throughput proteomics.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation.RNA (New York, N.Y.) · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In wild-type cells, the 3' poly(A) structure is necessary for translation of mRNA and for mRNA stability. The superkiller 2 (ski2), ski3, ski6, ski7, and ski8 mutations enhance the expression of the poly(A)(-) mRNAs of yeast RNA viruses. Ski2p is a DEVH-box RNA helicase and Slh1p resembles Ski2p. Both repress L-A double-stranded RNA (dsRNA) virus copy number, further suggesting that their functions may overlap. We find that slh1Delta ski2Delta double mutants are healthy (in the absence of viruses) and show normal rates of turnover of several cellular mRNAs. The slh1Delta ski2Delta strains translate electroporated nonpoly(A) mRNA with the same kinetics as polyA(+) mRNA. Thus, the translation apparatus is inherently capable of efficiently using nonpoly(A) mRNA even in the presence of normal amounts of competing poly(A)(+) mRNA, but is normally prevented from doing so by the combined action of the nonessential proteins Ski2p and Slh1p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.