ArticleThe Journal of physiology2000
Vacuole formation in fatigued skeletal muscle fibres from frog and mouse: effects of extracellular lactate.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- The Influence of Supplemental Dietary Linoleic Acid on Skeletal Muscle Contractile Function in a Rodent Model of Barth Syndrome.Frontiers in physiology · 2021Article
- Epinephrine augments posttetanic potentiation in mouse skeletal muscle with and without myosin phosphorylation.Physiological reports · 2018Article
- Influence of longitudinal radiation exposure from microcomputed tomography scanning on skeletal muscle function and metabolic activity in female CD-1 mice.Physiological reports · 2017Article
- Myosin light chain phosphorylation is required for peak power output of mouse fast skeletal muscle in vitro.Pflugers Archiv : European journal of physiology · 2016Article
- Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.Disease models & mechanisms · 2015Article
- The couplonopathies: A comparative approach to a class of diseases of skeletal and cardiac muscle.The Journal of general physiology · 2015Review
- The determinants of transverse tubular volume in resting skeletal muscle.The Journal of physiology · 2014Article
- Usage of a localised microflow device to show that mitochondrial networks are not extensive in skeletal muscle fibres.PloS one · 2014Article
- Tetanic force potentiation of mouse fast muscle is shortening speed dependent.Journal of muscle research and cell motility · 2012Article
- Longitudinal and transversal propagation of excitation along the tubular system of rat fast-twitch muscle fibres studied by high speed confocal microscopy.The Journal of physiology · 2012Article
- Dysferlin, annexin A1, and mitsugumin 53 are upregulated in muscular dystrophy and localize to longitudinal tubules of the T-system with stretch.Journal of neuropathology and experimental neurology · 2011Article
- Posttetanic potentiation in mdx muscle.Journal of muscle research and cell motility · 2010Article
- Kinetics of contraction-induced GLUT4 translocation in skeletal muscle fibers from living mice.Diabetes · 2010Article
- Local calcium signals induced by hyper-osmotic stress in mammalian skeletal muscle cells.Journal of muscle research and cell motility · 2009Article
- The accessibility and interconnectivity of the tubular system network in toad skeletal muscle.The Journal of physiology · 2008Article
- Acetazolamide prevents vacuolar myopathy in skeletal muscle of K(+) -depleted rats.British journal of pharmacology · 2008Article
- Alterations in triad ultrastructure following repetitive stimulation and intracellular changes associated with exercise in amphibian skeletal muscle.Journal of muscle research and cell motility · 2007Article
- Osmotic properties of the sealed tubular system of toad and rat skeletal muscle.The Journal of general physiology · 2004Article
- Development of T-tubular vacuoles in eccentrically damaged mouse muscle fibres.The Journal of physiology · 2002Article
- Excitation-contraction coupling and fatigue mechanisms in skeletal muscle: studies with mechanically skinned fibres.Journal of muscle research and cell motility · 2002Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated, living muscle fibres from either Xenopus or mouse were observed in a confocal microscope and t-tubules were visualized with sulforhodamine B. Observations were made before and after fatiguing stimulation. In addition, experiments were performed on fibres observed in an ordinary light microscope with dark-field illumination. In Xenopus fibres, recovering after fatigue, t-tubules started to show dilatations 2-5 min post-fatigue. These swellings increased in size over the next 10-20 min to form vacuoles. After 2-3 h of recovery the appearance of the fibres was again normal and force production, which had been markedly depressed 10-40 min post-fatigue, was close to control. Vacuoles were not observed in mouse fibres, fatigued with the same protocol and allowed to recover. In Xenopus fibres, fatigued in normal Ringer solution and allowed to recover in Ringer solution with 30-50 mM L-lactate substituting for chloride (lactate-Ringer), the number and size of vacuoles were markedly reduced. Also, force recovery was significantly faster. Replacement of chloride by methyl sulphate or glucuronate had no effect on vacuolation. Resting Xenopus fibres exposed to 50 mM lactate-Ringer and transferred to normal Ringer solution displayed vacuoles within 5-10 min, but to a smaller extent than after fatigue. Vacuolation was not associated with marked force reduction. Mouse fibres, fatigued in 50 mM lactate-Tyrode (L-lactate substituting for chloride in Tyrode solution) and recovering in normal Tyrode solution, displayed vacuoles for a limited period post-fatigue. Vacuolation had no effect on force production. The results are consistent with the view that lactate, formed during fatigue, is transported into the t-tubules where it attracts water and causes t-tubule swelling and vacuolation. This vacuolation may be counteracted in vivo due to a gradual extracellular accumulation of lactate during fatigue.
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