ArticleThe Journal of physiology2000
Sarcomeric Ca2+ gradients during activation of frog skeletal muscle fibres imaged with confocal and two-photon microscopy.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Honey, they shrunk the ATP.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- A stochastic simulation of skeletal muscle calcium transients in a structurally realistic sarcomere model using MCell.PLoS computational biology · 2019Article
- Store-operated CaCommunications biology · 2018Article
- Article
- Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140.The Journal of general physiology · 2016Article
- Intracellular calcium movements during relaxation and recovery of superfast muscle fibers of the toadfish swimbladder.The Journal of general physiology · 2014Article
- Comparison of myoplasmic calcium movements during excitation-contraction coupling in frog twitch and mouse fast-twitch muscle fibers.The Journal of general physiology · 2013Article
- Intracellular calcium movements during excitation-contraction coupling in mammalian slow-twitch and fast-twitch muscle fibers.The Journal of general physiology · 2012Review
- Calcium indicators and calcium signalling in skeletal muscle fibres during excitation-contraction coupling.Progress in biophysics and molecular biology · 2011Review
- Effects of membrane depolarization and changes in extracellular [K(+)] on the Ca (2+) transients of fast skeletal muscle fibers. Implications for muscle fatigue.Journal of muscle research and cell motility · 2010Article
- Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.Biophysical journal · 2009Article
- Evolution and modulation of intracellular calcium release during long-lasting, depleting depolarization in mouse muscle.The Journal of physiology · 2008Article
- Micropit: A New Cell Culturing Approach for Characterization of Solitary Astrocytes and Small Networks of these Glial Cells.Frontiers in neuroengineering · 2008Article
- Simulation of Ca2+ movements within the sarcomere of fast-twitch mouse fibers stimulated by action potentials.The Journal of general physiology · 2007Article
- A mathematical model of fatigue in skeletal muscle force contraction.Journal of muscle research and cell motility · 2007Article
- Three-dimensional distribution of ryanodine receptor clusters in cardiac myocytes.Biophysical journal · 2006Article
- Calcium release domains in mammalian skeletal muscle studied with two-photon imaging and spot detection techniques.The Journal of general physiology · 2006Article
- Confocal imaging of [Ca2+] in cellular organelles by SEER, shifted excitation and emission ratioing of fluorescence.The Journal of physiology · 2005Article
- Comparison between the predictions of diffusion-reaction models and localized Ca2+ transients in amphibian skeletal muscle fibers.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intra-sarcomeric gradients of [Ca2+] during activation of action potential stimulated frog single fibres were investigated with the Ca2+ indicator fluo-3 and confocal and two-photon microscopy. The object of these experiments was to look for evidence of extra-junctional Ca2+ release and examine the microscopic diffusion of Ca2+ within the sarcomere. By exploiting the spatial periodicity of sarcomeres within the fibre, we could achieve a high effective line-scanning rate ( approximately 8000 lines s-1), although the laser scanning microscope was limited to < 1000 lines s-1. At this high time resolution, the time course of fluorescence changes was very different at the z- and m-lines, with a significant delay ( approximately 1 ms; 22 C) between the rise of fluorescence at the z-line and the m-line. To calculate the expected fluorescence changes, we used a multi-compartment model of Ca2+ movements in the half-sarcomere in which Ca2+ release was restricted to triadic junctions (located at z-lines). Optical blurring by the microscope was simulated to generate fluorescence signals which could be compared directly to experimental data. The model which reproduced our experimental findings most accurately included Ca2+ binding by ATP, as well as indicator binding to immobile sarcomeric proteins. After taking sarcomeric misregistration within the fibre into account, there was very good agreement between the model and experimental results. We conclude that there is no experimental evidence for Ca2+ release at locations other than at z-lines. In addition, our calculations support the conclusion that rapidly diffusing Ca2+ buffers (such as ATP) are important in shaping the Ca2+ transient and that the details of intracellular indicator binding need to be considered to explain correctly the time course of fluorescence change in the fibre.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.