ArticleJournal of clinical microbiology2000
Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 67 citations in OpenAlex.
- Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area.Frontiers in veterinary science · 2025Article
- Tuberculosis Epidemiology and Spatial Ecology at the Cattle-Wild Boar Interface in Northern Spain.Transboundary and emerging diseases · 2023Article
- Prevalence ofAnimals : an open access journal from MDPI · 2022Article
- Pathological, Histological, and Molecular Based Investigations Confirm NovelBioMed research international · 2022Article
- Histopathological Investigations and Molecular Confirmation RevealBioMed research international · 2022Article
- Spatial and Temporal Distribution ofAnimals : an open access journal from MDPI · 2021Article
- Immunoinformatic-Based Prediction of Candidate Epitopes for the Diagnosis and Control of Paratuberculosis (Johne's Disease).Pathogens (Basel, Switzerland) · 2020Article
- Tuberculosis Epidemiology and Badger (Pathogens (Basel, Switzerland) · 2019Article
- Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques.Veterinary world · 2016Article
- Standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover Mycobacterium tuberculosis complex members.Frontiers in molecular biosciences · 2014Review
- Wild boar tuberculosis in Iberian Atlantic Spain: a different picture from Mediterranean habitats.BMC veterinary research · 2013Article
- Enhanced detection of tuberculous mycobacteria in animal tissues using a semi-nested probe-based real-time PCR.PloS one · 2013Article
- Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis.Veterinary medicine international · 2010Article
- Single nucleotide polymorphisms in the IS900 sequence of Mycobacterium avium subsp. paratuberculosis are strain type specific.Journal of clinical microbiology · 2009Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Polymerase chain reaction (PCR) amplification of IS6110 sequences to detect Mycobacterium tuberculosis complex from formalin-fixed paraffin-embedded tissues of deer (Axis axis).Veterinary research communications · 2007Article
- New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis.BMC microbiology · 2006Article
- Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk.Applied and environmental microbiology · 2005Article
- Definitive differentiation between single and mixed mycobacterial infections in red deer (Cervus elaphus) by a combination of duplex amplification of p34 and f57 sequences and Hpy188I enzymatic restriction of duplex amplicons.Journal of clinical microbiology · 2005Article
- Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine.Clinical microbiology reviews · 2001Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously isolated and sequenced two genomic segments of Mycobacterium avium subsp. paratuberculosis, namely, f57, a species-specific sequence, and the p34 gene, coding for a 34-kDa antigenic protein. Comparison of sequences upstream of the p34 open reading frame (us-p34) from M. avium subsp. paratuberculosis and M. tuberculosis showed a 79-base deletion in M. tuberculosis. Sequence analysis of the p34 genes in another two species, M. bovis (strain BCG) and M. avium (strain D4), confirmed the differences observed between tuberculous and nontuberculous species. A duplex diagnostic PCR strategy based on coamplification of nonhomologous us-p34 and species-specific f57 sequences was therefore developed. Duplex PCR yielded three different patterns, specific either for tuberculous bacilli (M. tuberculosis, M. bovis, and M. africanum), for both nontuberculous mycobacteria M. avium and M. intracellulare, or for M. avium subsp. paratuberculosis. The specificity of this single-step DNA-based assay was assessed on DNA from cultured mycobacterial strains, as well as on a panel of formalin-fixed and paraffin-embedded tissues from cattle. Molecular assay results from tissular DNA were compared to conventional bacteriological and histological test results, including those obtained by Ziehl-Neelsen staining on tissue biopsy specimens. Molecular discrimination was successful and confirmed the value of duplex us-p34 and f57 sequence amplification for differential diagnosis of tuberculosis, paratuberculosis, or infections caused by other members of the M. avium complex.
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