ArticleJournal of clinical microbiology2000
Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 128 citations in OpenAlex.
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Toward sustainable diagnostics forRSC advances · 2026Review
- Cytosolic nucleic acid sensing as driver of critical illness: mechanisms and advances in therapy.Signal transduction and targeted therapy · 2025Review
- Rapid and simple detection ofFrontiers in cellular and infection microbiology · 2025Article
- Prevalence and drug susceptibility of clinicalOne health (Amsterdam, Netherlands) · 2024Article
- Development of a Simple DNA Extraction Method andPathogens (Basel, Switzerland) · 2022Article
- Chromogenic medium versus PCR-RFLP in the speciation of Candida: a comparative study.BMC research notes · 2019Article
- Article
- Improved detection of deeply invasive candidiasis with DNA aptamers specific binding to (1→3)-β-D-glucans from Candida albicans.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- The Role of PCR in the Diagnosis of Candida Vulvovaginitis-a New Gold Standard?Current infectious disease reports · 2015Article
- Neonatal candidiasis: diagnosis, prevention, and treatment.The Journal of infection · 2014Review
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- Evaluation of chromogenic media and seminested PCR in the identification of Candida species.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Visual analysis of DNA microarray data for accurate molecular identification of non-albicans Candida isolates from patients with candidemia episodes.Journal of clinical microbiology · 2013Article
- Candida osteomyelitis in a gelding.The Canadian veterinary journal = La revue veterinaire canadienne · 2013Article
- Candida infections and their prevention.ISRN preventive medicine · 2013Review
- Pro-inflammatory action of Candida albicans DNA in zymosan-induced arthritis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Isolation and identification of Candida from the oral cavity.ISRN dentistry · 2011Article
- Identification of Candida spp. by phenotypic tests and PCR.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.The Journal of molecular diagnostics : JMD · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis. Universal primer sequences used in the PCR assay are derived from the internal transcribed spacer rRNA gene of fungi, whereas the biotinylated hybridization probe used in a DNA enzyme immunoassay (DEIA) binds specifically to C. albicans DNA. The sensitivity of this PCR-DEIA method is very high; the detection limit for genomic Candida DNA is one C. albicans genome per assay. Blood from uninfected and infected persons, ranging from healthy volunteers, patients with mucocutaneous infections, and patients at risk to develop a systemic Candida infection to patients with an established systemic candidiasis, was analyzed for the presence of C. albicans to diagnose fungal infection. Candida DNA could not be detected in sera of 16 culture-negative controls and from 11 nonsystemic candidal infections by PCR or DEIA. Blood cultures from patients at risk were all negative for Candida, whereas all blood cultures from systemic candidiasis patients were positive. However, Candida DNA could be detected by PCR and DEIA in the serum from three out of nine patients who were at risk for a systemic infection and in the serum of all seven patients who had already developed an invasive Candida infection. PCR is more sensitive than blood culture, since some of the patients at risk for invasive yeast infection, whose blood cultures were all negative for Candida, tested positive in the PCR amplification. These results indicate the potential value of PCR for detecting C. albicans in serum samples and for identifying patients at risk for invasive candidiasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.