ArticleJournal of clinical microbiology2000
Detection of Paracoccidioides brasiliensis in tissue samples by a nested PCR assay.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 94 citations in OpenAlex.
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Mycological diagnosis of paracoccidioidomycosis in a hospital from a nonendemic area: classical and molecular methodsBiomedica : revista del Instituto Nacional de Salud · 2023Article
- Molecular Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Paracoccidioidomycosis: Current Status and Future Trends.Clinical microbiology reviews · 2022Review
- Accompanying a semi-nested PCR assay to support histopathology findings of fungal keratitis in formalin-fixed paraffin-embedded corneal samples.Journal of clinical laboratory analysis · 2022Article
- PbGP43 Genotyping Using Paraffin-Embedded Biopsies of Human Paracoccidioidomycosis Reveals a Genetically Distinct Lineage in the Paracoccidioides brasiliensis Complex.Mycopathologia · 2022Article
- Evaluating a semi-nested PCR to support histopathology reports of fungal rhinosinusitis in formalin-fixed paraffin-embedded tissue samples.Journal of clinical laboratory analysis · 2022Article
- Molecular Tools for Detection and Identification ofJournal of fungi (Basel, Switzerland) · 2020Review
- Article
- Case Report: Misleading Serological Diagnosis of Paracoccidioidomycosis in a Young Patient with the Acute Form Disease:The American journal of tropical medicine and hygiene · 2018Article
- Paracoccidioidomycosis: Current Perspectives from Brazil.The open microbiology journal · 2017Review
- Molecular Diagnosis of Pathogenic Sporothrix Species.PLoS neglected tropical diseases · 2015Article
- PARACOCCIDIOIDOMYCOSIS TREATMENT.Revista do Instituto de Medicina Tropical de Sao Paulo · 2015Review
- Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report.The journal of venomous animals and toxins including tropical diseases · 2014Article
- Unifocal bone paracoccidioidomycosis, Brazil.The American journal of tropical medicine and hygiene · 2012Article
- Application of PCR in serum samples for diagnosis of paracoccidioidomycosis in the southern Bahia-Brazil.PLoS neglected tropical diseases · 2012Article
- PCR based identification and discrimination of agents of mucormycosis and aspergillosis in paraffin wax embedded tissue.Journal of clinical pathology · 2005Article
- Primers for clinical detection of Paracoccidioides brasiliensis.Journal of clinical microbiology · 2005Article
- Atypical serological response marked by a lack of detectable anti-gp43 antibodies in a patient with disseminated paracoccidioidomycosis.Journal of clinical microbiology · 2005Article
- Amplification of coccidioidal DNA in clinical specimens by PCR.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nested PCR assay for the detection of Paracoccidioides brasiliensis DNA was evaluated, using a sequence of the immunogenic gp43 gene as a target. This gene encodes an outer membrane protein unique to this dimorphic fungus. DNA from six clinical isolates and the ATCC strain 60885 of P. brasiliensis, as well as DNA from closely related fungi, was examined to determine detection limits and cross-reactions. PCR was done on DNA extracts of lung homogenates from 23 experimentally P. brasiliensis-infected and two uninfected BALB/c mice and from 20 Histoplasma capsulatum-infected ICR mice. The results were compared to quantitative cultures. A detection limit of 0.5 fg of specific DNA was determined using cloned plasmid DNA. In all seven P. brasiliensis isolates, the expected 196-bp nested PCR product was found. Their sequences were 100% identical to the gp43 gene sequence in GenBank. DNA extracts of all other, related fungi were negative. The PCR assay was positive in 21 out of 23 culture-positive lung homogenates with concentrations of 1 x 10(3) to 1.3 x 10(7) CFU of P. brasiliensis per g of lung. Uninfected BALB/c mice and H. capsulatum-infected mice samples gave negative results. The high sensitivity and specificity of this new nested PCR assay for the detection of P. brasiliensis in tissue samples were demonstrated. The assay may be useful for diagnosis in human tissue samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.