ArticleJournal of clinical microbiology2000
Rapid detection and identification of Candida, Aspergillus, and Fusarium species in ocular samples using nested PCR.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02850653 (Contribution of Fast Molecular Bacterial Identification by Real-time PCR in Managing of Postoperative Acute Endophthalmitis), which is not on this map. Cited by 41 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Contribution of Fast Molecular Bacterial Identification by Real-time PCR in Managing of Postoperative Acute Endophthalmitis
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 171 citations in OpenAlex.
- Aspergillus Polymerase Chain Reaction: Systematic Review of Evidence for Clinical Use in Comparison With Antigen Testing.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Pooled it
- International consensuses and guidelines on diagnosing and managing fungal endophthalmitis by the Asia-Pacific Vitreo-retina Society (APVRS), the Academy of the Asia-Pacific Professors of Ophthalmology (AAPPO), and the Asia-Pacific Society of Ocular Inflammation and Infection (APSOII).Eye and vision (London, England) · 2025Review
- Evaluation of the potential for diagnosis of fungal keratitis using a Fusarium-specific antibody.Scientific reports · 2025Article
- First Report ofPathogens (Basel, Switzerland) · 2025Article
- Clinical presentation and outcome of patients with endogenous endophthalmitis.International ophthalmology · 2025Article
- An Evaluation of the OLMJournal of fungi (Basel, Switzerland) · 2022Article
- Assessment of genotyping markers in the molecular characterization of a population of clinical isolates of Fusarium in Colombia.Biomedica : revista del Instituto Nacional de Salud · 2022Article
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- Article
- Rapid Isolation and Concentration of Pathogenic Fungi Using Inertial Focusing on a Chip-Based Platform.Frontiers in cellular and infection microbiology · 2019Article
- Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities ofBioMed research international · 2019Article
- A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases.Japanese journal of ophthalmology · 2017Review
- Development and Evaluation of a Molecular Diagnostic Method for Rapid Detection of Histoplasma capsulatum var. farciminosum, the Causative Agent of Epizootic Lymphangitis, in Equine Clinical Samples.Journal of clinical microbiology · 2016Article
- Study of the prevalence and association of ocular chlamydial conjunctivitis in women with genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans attending outpatient clinic.International journal of ophthalmology · 2016Article
- Endogenous endophthalmitis: diagnosis, management, and prognosis.Journal of ophthalmic inflammation and infection · 2015Article
- Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis.Current medical mycology · 2015Article
- Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction.Jundishapur journal of microbiology · 2015Article
- Etiological agents of fungal endophthalmitis: diagnosis and management.International ophthalmology · 2014Review
- Murine model of disseminated fusariosis: evaluation of the fungal burden by traditional CFU and quantitative PCR.Mycopathologia · 2013Article
- Novel diagnosis of fungal endophthalmitis by broad-range real-time PCR detection of fungal 28S ribosomal DNA.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
A protocol for the rapid detection of fungal DNA in ocular samples, derived from three species, Candida albicans, Aspergillus fumigatus, and Fusarium solani, has been developed. Two novel panfungal primers complementary to 18S rRNA sequences present in all three species were designed. Panfungal PCR was followed by three nested PCRs utilizing species-specific primers. PCR sensitivity ranged from 50 to 100 fg of free DNA and between one and two C. albicans organisms. In addition, we also developed a rapid and reliable DNA extraction protocol. This protocol minimized DNA loss during extraction, whilst removing compounds from vitreous and aqueous fluids that have previously been shown to have inhibitory effects on PCR. Preliminary results obtained after testing the protocol on three patient samples support culture results and medical history. However, one patient was PCR positive but culture negative, suggesting that the sensitivity of this protocol may exceed that of traditional culture techniques. This system, therefore, constitutes an additional protocol that may significantly aid patient management in cases where fungal endophthalmitis is suspected.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.