ArticleJournal of clinical microbiology2000
Relative abundance of oligosaccharides in Candida species as determined by fluorophore-assisted carbohydrate electrophoresis.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
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- Release of protein N-glycans by effectors of a Hofmann carboxamide rearrangement.Frontiers in molecular biosciences · 2022Article
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- Enzymatic Conversion of Mannan-Rich Plant Waste Biomass into Prebiotic Mannooligosaccharides.Foods (Basel, Switzerland) · 2021Review
- Role of Protein Mannosylation in theFrontiers in microbiology · 2019Article
- Cell wall mannan of Candida krusei mediates dendritic cell apoptosis and orchestrates Th17 polarization via TLR-2/MyD88-dependent pathway.Scientific reports · 2018Article
- Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction.Frontiers in microbiology · 2016Article
- Disruption of Protein Mannosylation AffectsFrontiers in microbiology · 2016Article
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- Innate inflammatory response and immunopharmacologic activity of micafungin, caspofungin, and voriconazole against wild-type and FKS mutant Candida glabrata isolates.Antimicrobial agents and chemotherapy · 2015Article
- β-1,2-Mannosyltransferases 1 and 3 Participate in Yeast and Hyphae O- and N-Linked Mannosylation and Alter Candida albicans Fitness During Infection.Open forum infectious diseases · 2015Article
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- Deficient beta-mannosylation of Candida albicans phospholipomannan affects the proinflammatory response in macrophages.PloS one · 2013Article
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- Horizontal transmission of Candida albicans and evidence of a vaccine response in mice colonized with the fungus.PloS one · 2011Article
- Muropeptide rescue in Bacillus subtilis involves sequential hydrolysis by beta-N-acetylglucosaminidase and N-acetylmuramyl-L-alanine amidase.Journal of bacteriology · 2010Article
- Synthetic glycopeptide vaccines combining beta-mannan and peptide epitopes induce protection against candidiasis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Capsule structural heterogeneity and antigenic variation in Cryptococcus neoformans.Eukaryotic cell · 2007Article
- Advances in combating fungal diseases: vaccines on the threshold.Nature reviews. Microbiology · 2007Review
- Surface hydrophobicity changes of two Candida albicans serotype B mnn4delta mutants.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal aldehydes of oligomannosyl residues released by hydrolysis were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) and separated with high resolution on the basis of size by polyacrylamide gel electrophoresis. ANTS fluorescence labeling was not biased by oligomannoside length; therefore, band fluorescence intensity was directly related to the relative abundance of individual oligomannoside moieties in heterogeneous samples. FACE analysis revealed the major oligomannosides released by acid hydrolysis and beta-elimination of Fehling-precipitated mannan from Candida albicans, which were the same as those previously reported in studies based on mass and nuclear magnetic spectroscopic analysis. FACE was also amenable to the analysis of samples obtained by direct hydrolysis of whole yeast cells. Whole-cell acid hydrolysis and whole-cell beta-elimination of two isolates each of C. albicans, C. glabrata, C. krusei, C. lusitaniae, C. parapsilosis, C. rugosa, C. stellatoidea, and C. tropicalis resulted in oligomannoside gel banding patterns that were species and strain specific for the 16 isolates surveyed. Whereas some bands were specific for an individual isolate or species, other bands were shared by two or three species in various groupings. Differences in the mannoprotein composition of C. albicans A9 and four spontaneous cell surface mutants were also detected. Mannan "fingerprints," or banding pattern profiles, derived from the electrophoretic mobilities of individual bands relative to the migration of acid-hydrolyzed dextran (relative migration index) yielded profiles characteristic of individual isolates not revealed by standard assimilation and biochemical profiles. FACE represents an accessible, sensitive, and quantitative analytical tool enabling the characterization of yeast mannan complexity.
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