ArticleJournal of clinical microbiology2000
Rapid identification of Candida dubliniensis using a species-specific molecular beacon.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 79 citations in OpenAlex.
- Detection ofJournal of medical microbiology · 2018Article
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- Molecular beacons: a novel optical diagnostic tool.Archivum immunologiae et therapiae experimentalis · 2013Review
- A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- Effect of antifungal therapy timing on mortality in cancer patients with candidemia.Antimicrobial agents and chemotherapy · 2010Article
- High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.The Journal of molecular diagnostics : JMD · 2010Article
- Molecular beacons in biomedical detection and clinical diagnosis.International journal of clinical and experimental pathology · 2008Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Use of a suspension array for rapid identification of the varieties and genotypes of the Cryptococcus neoformans species complex.Journal of clinical microbiology · 2005Article
- An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems.BMC microbiology · 2005Article
- Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole.Journal of clinical microbiology · 2005Article
- Molecular characterization of fluconazole resistance in a case of Candida albicans ocular infection.Journal of clinical microbiology · 2004Article
- Discrimination between Candida albicans and Candida dubliniensis isolated from HIV-positive patients by using commercial method in comparison with PCR assay.Folia microbiologica · 2004Article
- Use of amplified fragment length polymorphism analysis to identify medically important Candida spp., including C. dubliniensis.Journal of clinical microbiology · 2003Article
- Evaluation of a method for identification of Candida dubliniensis bloodstream isolates.Journal of clinical microbiology · 2003Article
- Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.Journal of clinical microbiology · 2003Article
- Rapid identification and differentiation of Candida albicans and Candida dubliniensis by capillary-based amplification and fluorescent probe hybridization.Journal of clinical microbiology · 2002Article
- Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2).BMC microbiology · 2002Article
- Identification of four distinct genotypes of Candida dubliniensis and detection of microevolution in vitro and in vivo.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida dubliniensis is an opportunistic fungal pathogen that has been linked to oral candidiasis in AIDS patients, although it has recently been isolated from other body sites. DNA sequence analysis of the internal transcribed spacer 2 (ITS2) region of rRNA genes from reference Candida strains was used to develop molecular beacon probes for rapid, high-fidelity identification of C. dubliniensis as well as C. albicans. Molecular beacons are small nucleic acid hairpin probes that brightly fluoresce when they are bound to their targets and have a significant advantage over conventional nucleic acid probes because they exhibit a higher degree of specificity with better signal-to-noise ratios. When applied to an unknown collection of 23 strains that largely contained C. albicans and a smaller amount of C. dubliniensis, the species-specific probes were 100% accurate in identifying both species following PCR amplification of the ITS2 region. The results obtained with the molecular beacons were independently verified by random amplified polymorphic DNA analysis-based genotyping and by restriction enzyme analysis with enzymes BsmAI and NspBII, which cleave recognition sequences within the ITS2 regions of C. dubliniensis and C. albicans, respectively. Molecular beacons are promising new probes for the rapid detection of Candida species.
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