ArticleThe EMBO journal2000
Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Drug Development Targeting the Ubiquitin-Proteasome System (UPS) for the Treatment of Human Cancers.Cancers · 2020Review
- Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.Nature neuroscience · 2019Article
- Proteasome Activity Profiling Uncovers Alteration of Catalytic β2 and β5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots.Frontiers in plant science · 2017Article
- The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.Oncogene · 2014Article
- BAG-6 is essential for selective elimination of defective proteasomal substrates.The Journal of cell biology · 2010Article
- Multitasking with ubiquitin through multivalent interactions.Trends in biochemical sciences · 2010Review
- The proteasome: overview of structure and functions.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2009Review
- Recognition and processing of ubiquitin-protein conjugates by the proteasome.Annual review of biochemistry · 2009Review
- Association of Rpn10 with high molecular weight complex is enhanced during retinoic acid-induced differentiation of neuroblastoma cells.Molecular and cellular biochemistry · 2007Article
- Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development.Molecular and cellular biology · 2007Article
- Scythe regulates apoptosis through modulating ubiquitin-mediated proteolysis of the Xenopus elongation factor XEF1AO.The Biochemical journal · 2007Article
- Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.Molecular biology of the cell · 2006Article
- Parkin binds the Rpn10 subunit of 26S proteasomes through its ubiquitin-like domain.EMBO reports · 2003Article
- The contribution of exon-skipping events on chromosome 22 to protein coding diversity.Genome research · 2001Article
- The cellular level of PR500, a protein complex related to the 19S regulatory particle of the proteasome, is regulated in response to stresses in plants.Molecular biology of the cell · 2001Article
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 26S proteasome is a multisubunit protein- destroying machinery that degrades ubiquitin-tagged proteins. To date only a single species of Rpn10, which possibly functions as a multiubiquitin chain-binding subunit, has been identified in various organisms. Here we report that mouse Rpn10 mRNAs occur in at least five distinct forms, named Rpn10a to Rpn10e, and that they are generated from a single gene by developmentally regulated, alternative splicing. Rpn10a is ubiquitously expressed, whereas Rpn10e is expressed only in embryos, with the highest levels of expression in the brain. Both forms of Rpn10 are components of the 26S proteasome, with an apparently similar affinity for multiubiquitylated [(125)I]lysozyme in vitro. However, they exert markedly divergent effects on the destruction of B-type cyclin in Xenopus egg extracts. Thus, the 26S proteasome occurs in at least two functionally distinct forms: one containing a ubiquitously expressed Rpn10a and the other a newly identified, embryo-specific Rpn10e. While the former is thought to perform proteolysis constitutively in a wide variety of cells, the latter may play a specialized role in early embryonic development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.