ArticleThe EMBO journal2000
Alternatively spliced hBRF variants function at different RNA polymerase III promoters.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- RNA polymerase III transcription as a disease factor.Genes & development · 2020Review
- BRF1 mutations alter RNA polymerase III-dependent transcription and cause neurodevelopmental anomalies.Genome research · 2015Article
- Different sequence signatures in the upstream regions of plant and animal tRNA genes shape distinct modes of regulation.Nucleic acids research · 2011Article
- Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells.BMC molecular biology · 2008Article
- Transcriptional regulation of human small nuclear RNA genes.Biochimica et biophysica acta · 2008Review
- The green tea component EGCG inhibits RNA polymerase III transcription.Biochemical and biophysical research communications · 2007Article
- Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2.International journal of biological sciences · 2007Article
- The p53 tumor suppressor protein represses human snRNA gene transcription by RNA polymerases II and III independently of sequence-specific DNA binding.Molecular and cellular biology · 2005Article
- Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing.Molecular and cellular biology · 2002Article
- Kinetic trapping of DNA by transcription factor IIIB.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
- A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The retinoblastoma tumor suppressor protein targets distinct general transcription factors to regulate RNA polymerase III gene expression.Molecular and cellular biology · 2000Article
- RNA polymerase III repression by the retinoblastoma tumor suppressor protein.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In yeast, a single form of TFIIIB is required for transcription of all RNA polymerase III (pol III) genes. It consists of three subunits: the TATA box-binding protein (TBP), a TFIIB-related factor, BRF, and B". Human TFIIIB is not as well defined and human pol III promoters differ in their requirements for this activity. A human homolog of yeast BRF was shown to be required for transcription at the gene-internal 5S and VA1 promoters. Whether or not it was also involved in transcription from the gene-external human U6 promoter was unclear. We have isolated cDNAs encoding alternatively spliced forms of human BRF that can complex with TBP. Using immunopurified complexes containing the cloned hBRFs, we show that while hBRF1 functions at the 5S, VA1, 7SL and EBER2 promoters, a different variant, hBRF2, is required at the human U6 promoter. Thus, pol III utilizes different TFIIIB complexes at structurally distinct promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.