ArticleProceedings of the National Academy of Sciences of the United States of America2000
Hyperpolarization-activated calcium channels at the tip of Arabidopsis root hairs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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73 citing papers in PubMed, 231 citations in OpenAlex.
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- MLO-mediated CaThe New phytologist · 2025Article
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
- Exogenous application of pectin triggers stomatal closure and immunity in Arabidopsis.Molecular plant pathology · 2024Article
- Calcium signaling in plant mineral nutrition: From uptake to transport.Plant communications · 2023Review
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- Low Concentration of Aluminum-Stimulated Pollen Tube Growth of Apples (Plants (Basel, Switzerland) · 2022Article
- ROOT HAIR DEFECTIVE 2 vesicular delivery to the apical plasma membrane domain during Arabidopsis root hair development.Plant physiology · 2022Article
- The signatures of organellar calcium.Plant physiology · 2021Review
- Benefits provided by four ectomycorrhizal fungi to Pinus taeda under different external potassium availabilities.Mycorrhiza · 2021Article
- P2K1 Receptor, Heterotrimeric Gα Protein and CNGC2/4 Are Involved in Extracellular ATP-Promoted Ion Influx in the Pollen ofPlants (Basel, Switzerland) · 2021Article
- Molecular Mechanisms and Biochemical Pathways for Micronutrient Acquisition and Storage in Legumes to Support Biofortification for Nutritional Security.Frontiers in plant science · 2021Review
- The CaFrontiers in plant science · 2021Article
- Calcium spikes, waves and oscillations in plant development and biotic interactions.Nature plants · 2020Review
- MgInternational journal of molecular sciences · 2020Article
- Elucidating the roles of three β-glucuronosyltransferases (GLCATs) acting on arabinogalactan-proteins using a CRISPR-Cas9 multiplexing approach in Arabidopsis.BMC plant biology · 2020Article
- Circadian gating of dark-induced increases in chloroplast- and cytosolic-free calcium in Arabidopsis.The New phytologist · 2020Article
- Three CNGC Family Members, CNGC5, CNGC6, and CNGC9, Are Required for Constitutive Growth ofPlant communications · 2020Article
- Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 inFrontiers in plant science · 2020Article
13 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The root hair elongative growth phase ("tip growth"), like that of other tip-growing systems such as pollen tubes, algal rhizoids, and fungal hyphae, is associated with an apex-high cytosolic free calcium ([Ca(2+)](c)) gradient generated by a local Ca(2+) influx at the tip. This gradient has been shown to be a fundamental regulator of tip growth. Here, we have performed patch-clamp experiments at root hair apices of Arabidopsis thaliana (after localized cell wall laser ablation) to characterize the plasma membrane Ca(2+) channels implicated in the tip Ca(2+) influx. We have identified a hyperpolarization-activated Ca(2+) conductance. This conductance is selective for Ca(2+) over K(+) and Cl(-) (P(Ca)/P(K) = 15; P(Ca)/P(Cl) = 25) and is fully blocked by < 100-microM trivalent cations (La(3+), Al(3+), Gd(3+)). The selectivity sequence among divalent cations (determined by comparisons of the channel unitary conductance) is Ba(2+) > Ca(2+) (22 pS in 10 mM) approximately Mg(2+) > Mn(2+). This conductance was operative at typical growing hair apical resting membrane potentials. Moreover, it was seen to be down-regulated in growing hair subapical regions, as well as at the tip of mature hairs (known not to exhibit Ca(2+) influx). We therefore propose that this inward-rectifying Ca(2+) conductance is inherently involved in the apical Ca(2+) influx of growing hairs. The observed enhancement of the conductance by increased [Ca(2+)](c) may form part of a positive feedback system for continued apical Ca(2+) influx during tip growth.
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