ArticleBiophysical journal2000
Multiple-particle tracking measurements of heterogeneities in solutions of actin filaments and actin bundles.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 216 citations in OpenAlex.
- Diffusing Wave Microrheology in Polymeric Fluids.Polymers · 2024Article
- Time-Dependent Material Properties of Aging Biomolecular Condensates from Different Viscoelasticity Measurements in Molecular Dynamics Simulations.The journal of physical chemistry. B · 2023Article
- 2D-GolgiTrack-a semi-automated tracking system to quantify morphological changes and dynamics of the Golgi apparatus and Golgi-derived membrane tubules.Medical & biological engineering & computing · 2022Article
- Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Particle tracking microrheology of cancer cells in living subjects.Materials today (Kidlington, England) · 2020Article
- Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.Acta biomaterialia · 2020Article
- Determination of rheology and surface tension of airway surface liquid: a review of clinical relevance and measurement techniques.Respiratory research · 2019Review
- Robust antigen-specific tuning of the nanoscale barrier properties of biogels using matrix-associating IgG and IgM antibodies.Acta biomaterialia · 2019Article
- Herpes simplex virus-binding IgG traps HSV in human cervicovaginal mucus across the menstrual cycle and diverse vaginal microbial composition.Mucosal immunology · 2018Article
- Rapid transport of deformation-tuned nanoparticles across biological hydrogels and cellular barriers.Nature communications · 2018Article
- Enhanced digestion inhibition and mucus penetration of F127-modified self-nanoemulsions for improved oral delivery.Asian journal of pharmaceutical sciences · 2018Article
- A blueprint for robust crosslinking of mobile species in biogels with weakly adhesive molecular anchors.Nature communications · 2017Article
- Active microrheology determines scale-dependent material properties of Chaetopterus mucus.PloS one · 2017Article
- Anti-PEG antibodies alter the mobility and biodistribution of densely PEGylated nanoparticles in mucus.Acta biomaterialia · 2016Article
- Microstructure, local dynamics, and flow behavior of colloidal suspensions with weak attractive interactions.Scientific reports · 2016Article
- Minimizing biases associated with tracking analysis of submicron particles in heterogeneous biological fluids.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Article
- IgG in cervicovaginal mucus traps HSV and prevents vaginal herpes infections.Mucosal immunology · 2014Article
- Remote magnetic actuation of micrometric probes for in situ 3D mapping of bacterial biofilm physical properties.Journal of visualized experiments : JoVE · 2014Article
- A microrheological study of hydrogel kinetics and micro-heterogeneity.The European physical journal. E, Soft matter · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
One of the central functions of actin cytoskeleton is to provide the mechanical support required for the establishment and maintenance of cell morphology. The mechanical properties of actin filament assemblies are a consequence of both the available polymer concentration and the actin regulatory proteins that direct the formation of higher order structures. By monitoring the displacement of well-dispersed microspheres via fluorescence microscopy, we probe the degree of spatial heterogeneity of F-actin gels and networks in vitro. We compare the distribution of the time-dependent mean-square displacement (MSD) of polystyrene microspheres imbedded in low- and high-concentration F-actin solutions, in the presence and absence of the F-actin-bundling protein fascin. The MSD distribution of a 2. 6-microM F-actin solution is symmetric and its standard deviation is similar to that of a homogeneous solution of glycerol of similar zero-shear viscosity. However, increasing actin concentration renders the MSD distribution wide and asymmetric, an effect enhanced by fascin. Quantitative changes in the shape of the MSD distribution correlate qualitatively with the presence of large heterogeneities in F-actin solutions produced by increased filament concentration and the presence of actin bundles, as detected by confocal microscopy. Multiple-particle tracking offers a new, quantitative method to characterize the organization of biopolymers in solution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.