ArticleBiophysical journal2000
Nonideality and the nucleation of sickle hemoglobin.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Discovery of Osivelotor (GBT021601): A Potent, Next-Generation Sickle Hemoglobin Polymerization Inhibitor.ACS medicinal chemistry letters · 2025Article
- Voxelotor does not inhibit sickle hemoglobin fiber formation upon complete deoxygenation.Biophysical journal · 2023Article
- The Sickle-Cell Fiber Revisited.Biomolecules · 2023Review
- Rational Drug Design of Peptide-Based Therapies for Sickle Cell Disease.Molecules (Basel, Switzerland) · 2019Review
- High-throughput assessment of hemoglobin polymer in single red blood cells from sickle cell patients under controlled oxygen tension.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Rapid and inefficient kinetics of sickle hemoglobin fiber growth.Science advances · 2019Article
- Dissecting the energies that stabilize sickle hemoglobin polymers.Biophysical journal · 2013Article
- Predicting the morphology of sickle red blood cells using coarse-grained models of intracellular aligned hemoglobin polymers.Soft matter · 2012Article
- Sodium metabisulfite-induced polymerization of sickle cell hemoglobin incubated in the extracts of three medicinal plants (Anacardium occidentale, Psidium guajava, and Terminalia catappa).Pharmacognosy magazine · 2011Article
- Band 3 catalyzes sickle hemoglobin polymerization.Biophysical chemistry · 2010Article
- Protein aggregation and protein instability govern familial amyotrophic lateral sclerosis patient survival.PLoS biology · 2008Article
- Metastable mesoscopic clusters in solutions of sickle-cell hemoglobin.Biophysical journal · 2007Article
- Heterogeneous nucleation in sickle hemoglobin: experimental validation of a structural mechanism.Biophysical journal · 2005Article
- Understanding the shape of sickled red cells.Biophysical journal · 2005Article
- Life in a crowded world.EMBO reports · 2004Article
- Liquid-liquid separation in solutions of normal and sickle cell hemoglobin.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Heterogeneous nucleation and crowding in sickle hemoglobin: an analytic approach.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homogeneous and heterogeneous nucleation kinetics of sickle hemoglobin (HbS) have been studied for various degrees of solution crowding by substitution of cross-linked hemoglobin A, amounting to 50% of the total hemoglobin. By cross-linking hemoglobin A, hybrid formation between hemoglobin A and hemoglobin S was prevented, thus simplifying the analysis of the results. Polymerization was induced by laser photolysis, and homogeneous nucleation kinetics were determined by observation of the stochastic behavior of the onset of light scattering. Heterogeneous nucleation was determined by observing the exponential growth of the progress curves, monitored by light scattering. At concentrations between 4 and 5 mM tetramer (i.e., approximately 30 g/dl), the substitution of 50% HbA for HbS slows the reaction by a factor of 10(3) to 10(4). Using scaled particle theory to account for the crowding of HbA, the observed decrease in the homogeneous nucleation rate was accurately predicted, with no variation of parameters required. Heterogeneous nucleation, on the other hand, is not well described in the present formulation, and the theory for this process appears to require modification of the way in which nonideality is introduced. Nonetheless, the accuracy of the homogeneous nucleation description suggests that such an approach may be useful for other assembly processes that occur in a crowded intracellular milieu.
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