ArticleBiophysical journal2000
ATP consumption and efficiency of human single muscle fibers with different myosin isoform composition.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers, 1 of them a synthesis that pooled it.
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Who cites it
129 citing papers in PubMed, 1 synthesis or guideline pooled it, 341 citations in OpenAlex.
- Effects of age on human skeletal muscle: a systematic review and meta-analysis of myosin heavy chain isoform protein expression, fiber size, and distribution.American journal of physiology. Cell physiology · 2024Pooled it
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- Effect of acute and short-term oral salbutamol treatments on maximal power output in non-asthmatic athletes.European journal of applied physiology · 2012Trial
- Skeletal muscle fatigue precedes the slow component of oxygen uptake kinetics during exercise in humans.The Journal of physiology · 2011Trial
- Interaction between muscle temperature and contraction velocity affects mechanical efficiency during moderate-intensity cycling exercise in young and older women.Journal of applied physiology (Bethesda, Md. : 1985) · 2009Trial
- Influence of muscle fibre type and pedal rate on the VO2-work rate slope during ramp exercise.European journal of applied physiology · 2004Trial
- Oxygen uptake kinetics during moderate, heavy and severe intensity "submaximal" exercise in humans: the influence of muscle fibre type and capillarisation.European journal of applied physiology · 2003Trial
- Diaphragmatic Mitochondrial Myopathy in a Patient-Derived Mouse Model of Barth Syndrome.Journal of developmental biology · 2026Article
- Isometric slow- and fast-contracting skeletal muscle fibres have more similar cellular and molecular properties at 37°C than at 25°C in older adults.The Journal of physiology · 2026Article
- Multiscale adaptations underlie bite force variation in rodents.Communications biology · 2026Article
- Skeletal Fiber Type in Muscle Pain and Dysfunction.Biomedicines · 2026Review
- The Oxygen Equivalent of Lactate Accumulation and Sex: Similar Work-Lactate Slopes in Men and Women Regardless of Body or Fat-Free Mass Scaling.FASEB bioAdvances · 2026Article
- Mental fatigue accompanied by whole-body surface cooling is associated with the impairment of subsequent endurance exercise performance.European journal of applied physiology · 2026Article
- Semaglutide-induced weight loss improves mitochondrial energy efficiency in skeletal muscle.Obesity (Silver Spring, Md.) · 2025Article
- Sprinters' and Marathon Runners' Performances Are Better Explained by Muscle Fibers' Percentage Cross-Sectional Area than Any Other Parameter of Muscle Fiber Composition.Sports (Basel, Switzerland) · 2025Article
- Jaw-Muscle Structure and Function in Primates: Insights Into Muscle Performance and Feeding-System Behaviors.Evolutionary anthropology · 2025Review
- Optimizing Resistance Training for Sprint and Endurance Athletes: Balancing Positive and Negative Adaptations.Sports medicine (Auckland, N.Z.) · 2024Review
- The highest work rate associated with a predominantly aerobic contribution coincides with the highest work rate at which VOEuropean journal of applied physiology · 2024Article
- A novel kinetic model to demonstrate the independent effects of ATP and ADP/Pi concentrations on sarcomere function.PLoS computational biology · 2024Article
- Disuse-Induced Muscle Fatigue: Facts and Assumptions.International journal of molecular sciences · 2024Review
69 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemomechanical transduction was studied in single fibers isolated from human skeletal muscle containing different myosin isoforms. Permeabilized fibers were activated by laser-pulse photolytic release of 1.5 mM ATP from p(3)-1-(2-nitrophenyl)ethylester of ATP. The ATP hydrolysis rate in the muscle fibers was determined with a fluorescently labeled phosphate-binding protein. The effects of varying load and shortening velocity during contraction were investigated. The myosin isoform composition was determined in each fiber by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. At 12 degrees C large variations (three- to fourfold) were found between slow and fast (2A and 2A-2B) fibers in their maximum shortening velocity, peak power output, velocity at which peak power is produced, isometric ATPase activity, and tension cost. Isometric tension was similar in all fiber groups. The ATP consumption rate increased during shortening in proportion to shortening velocity. At 12 degrees C the maximum efficiency was similar (0.21-0.27) for all fiber types and was reached at a higher speed of shortening for the faster fibers. In all fibers, peak efficiency increased to approximately 0.4 when the temperature was raised from 12 degrees C to 20 degrees C. The results were simulated with a kinetic scheme describing the ATPase cycle, in which the rate constant controlling ADP release is sensitive to the load on the muscle. The main difference between slow and fast fibers was reproduced by increasing the rate constant for the hydrolysis step, which was rate limiting at low loads. Simulation of the effect of increasing temperature required an increase in the force per cross-bridge and an acceleration of the rate constants in the reaction pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.